Efficient expression of a 100-kilodalton mosquitocidal toxin in protease-deficient recombinant Bacillus sphaericus release_yvzjyub3gfcb7jn2ynmw5i54bi

by T Thanabalu, A G Porter

Abstract

The expression of the 100-kDa mosquitocidal toxin (Mtx) during vegetative growth and sporulation in nine different mosquito-larvicidal strains of Bacillus sphaericus has been analyzed. In five out of the nine strains the 100-kDa toxin was found to be expressed predominantly in the vegetative phase of growth, and in all nine strains the level of the toxin in sporulated cells was very low or undetectable. Strains in four out of the six DNA homology groups of B. sphaericus produced intracellular and extracellular proteases, which degraded the 100-kDa toxin, during sporulation. The 100-kDa toxin gene was expressed by using its native promoter on a multicopy number plasmid in B. sphaericus 1693 (protease negative) and B. sphaericus 13052 (protease positive). High levels of the 100-kDa toxin were produced in vegetative cells of both strains as well as in sporulated cells of protease-negative strain 1693, which is in contrast to the low levels of the 100-kDa toxin produced in sporulated cells of protease-positive strain 13052. Thus, the small amount of the 100-kDa toxin in sporulated cells of the nine mosquito-larvicidal strains is probably due to degradation of the 100-kDa toxin synthesized during vegetative growth by a protease(s) produced during sporulation. B. sphaericus 1693 transformed with the 100-kDa toxin gene was as toxic to mosquito larvae during both vegetative growth and sporulation as the natural high-toxicity strains of sporulated B. sphaericus.(ABSTRACT TRUNCATED AT 250 WORDS)
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ISSN-L 0099-2240
Volume 61
Issue 11
Page(s) 4031-6
Release Year 1995
Primary Language en (lookup)

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Type  article-journal
Stage   published
Year   1995
PubMed  8526518
PMC  PMC167711
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