The Two-Component System GrvRS (EtaRS) RegulatesaceExpression in Enterococcus faecalis OG1RF release_yqlszg236ngddh3sz6rsrs5k3e

Abstract

Expression of<jats:italic>ace</jats:italic>(<jats:underline>a</jats:underline>dhesin to<jats:underline>c</jats:underline>ollagen of<jats:named-content content-type="genus-species"><jats:underline>E</jats:underline>nterococcus faecalis</jats:named-content>), encoding a virulence factor in endocarditis and urinary tract infection models, has been shown to increase under certain conditions, such as in the presence of serum, bile salts, urine, and collagen and at 46°C. However, the mechanism of<jats:italic>ace</jats:italic>/Ace regulation under different conditions is still unknown. In this study, we identified a two-component regulatory system GrvRS as the main regulator of<jats:italic>ace</jats:italic>expression under these stress conditions. Using Northern hybridization and β-galactosidase assays of an<jats:italic>ace</jats:italic>promoter-<jats:italic>lacZ</jats:italic>fusion, we found transcription of<jats:italic>ace</jats:italic>to be virtually absent in a<jats:italic>grvR</jats:italic>deletion mutant under the conditions that increase<jats:italic>ace</jats:italic>expression in wild-type OG1RF and in the complemented strain. Moreover, a<jats:italic>grvR</jats:italic>mutant revealed decreased collagen binding and biofilm formation as well as attenuation in a murine urinary tract infection model. Here we show that GrvR plays a major role in control of<jats:italic>ace</jats:italic>expression and<jats:named-content content-type="genus-species">E. faecalis</jats:named-content>virulence.
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