In vitro conservation of Piper aduncum and Piper hispidinervum under slow-growth conditions release_vggodwsrxfho3j5756l5ck6pmu

by Tatiane Loureiro da Silva, Jonny Everson Scherwinski-Pereira

Published in Pesquisa Agropecuária Brasileira by FapUNIFESP (SciELO).

Volume 46p384-389 (2011)

Abstract

The objective of this work was to evaluate in vitro storage of Piper aduncum and P. hispidinervum under slow-growth conditions. Shoots were stored at low temperatures (10, 20 and 25°C), and the culture medium was supplemented with osmotic agents (sucrose and mannitol - at 1, 2 and 3%) and abiscisic acid - ABA (0, 0.5, 1.0, 2.0 and 3.0 mg L-1). After six-months of storage, shoots were evaluated for survival and regrowth. Low temperature at 20ºC was effective for the in vitro conservation of P. aduncum and P. hispidinervum shoots. In vitro cultures maintained at 20ºC on MS medium showed 100% survival with slow-growth shoots. The presence of mannitol or ABA, in the culture medium, negatively affected shoot growth, which is evidenced by the low rate of recovered shoots.
In application/xml+jats format

Archived Files and Locations

application/pdf  490.5 kB
file_3j4hwjchyfbw3ebhdxcpize4y4
web.archive.org (webarchive)
web.archive.org (webarchive)
www.scielo.br (repository)
www.alice.cnptia.embrapa.br (web)
Read Archived PDF
Archived
Type  article-journal
Stage   published
Year   2011
Container Metadata
Open Access Publication
In DOAJ
In ISSN ROAD
In Keepers Registery
ISSN-L:  0100-204X
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: f6381f5c-0643-4198-b041-999de3abf401
API URL: JSON