Spatially-Distinct Redox Conditions and Degradation Rates Following Field-Scale Bioaugmentation for RDX-Contaminated Groundwater Remediation release_ubqeqqe6pbd7bfjupvj4mlkbyq

by M.M. Michalsen, A.S. King, J.D. Istok, F.H. Crocker, Mark Fuller, Kate Kucharzyk, M.J. Gander

Published in Journal of Hazardous Materials by Elsevier BV.

2019   Volume 387, p121529

Abstract

In situ bioaugmentation for cleanup of an hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)-contaminated groundwater plume was recently demonstrated. Results of a forced-gradient, field-scale cell transport test with Gordonia sp. KTR9 and Pseudomonas fluorescens strain I-C cells (henceforth "KTR9" and "Strain I-C") showed these strains were transported 13 m downgradient over 1 month. Abundances of xplA and xenB genes, respective indicators of KTR9 and Strain I-C, approached injection well cell densities at 6 m downgradient, whereas gene abundances (and conservative tracer) had begun to increase at 13 m downgradient at test conclusion. In situ push-pull tests were subsequently completed to measure RDX degradation rates in the bioaugmented wells under ambient gradient conditions. Time-series monitoring of RDX, RDX end-products, conservative tracer, xplA and xenB gene copy numbers and XplA and XenB protein abundance were used to assess the efficacy of bioaugmentation and to estimate the apparent first-order RDX degradation rates during each test. A collective evaluation of redox conditions, RDX end-products, varied RDX degradation kinetics, and biomarkers indicated that Strain I-C and KTR9 rapidly degraded RDX. Results showed bioaugmentation is a viable technology for accelerating RDX cleanup in the demonstration site aquifer and may be applicable to other sites. Full-scale implementation considerations are discussed.
In text/plain format

Archived Content

There are no accessible files associated with this release. You could check other releases for this work for an accessible version.

"Dark" Preservation Only
Save Paper Now!

Know of a fulltext copy of on the public web? Submit a URL and we will archive it

Type  article-journal
Stage   published
Date   2019-11-23
Language   en ?
Journal Metadata
Not in DOAJ
In Keepers Registry
ISSN-L:  0304-3894
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: 977d704f-7065-4cef-907c-97ce7537a21f
API URL: JSON