Fluorescence correlation spectroscopy (IUPAC Technical Report)
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by
Jörg Enderlein
Abstract
We present an overview on the applicability of fluorescence correlation
spectroscopy (FCS) for the accurate determination of translational diffusion
coefficients and thus, via the Stokes–Einstein relation, of molecular size. We
consider several of the most common sources of optical aberrations and their
impact on the outcome of conventional FCS measurements. We describe also a new
variant of FCS, dual-focus FCS, which is robust against most of the considered
aberrations, and we report reference values of diffusion coefficients for
several fluorescent dyes across the visible spectrum.
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article-journal
Stage
published
Date 2013-04-02
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