Three dimensional electron microscopy and in silico tools for macromolecular structure determination
release_sny5lwvfnbckhpk3n2anh7dx7q
by
Subhomoi Borkotoky, Chetan Kumar Meena, Mohammad Wahab Khan, Ayaluru Murali
2013 Volume 12, p335-46
Abstract
Recently, structural biology witnessed a major tool - electron microscopy - in solving the structures of macromolecules in addition to the conventional techniques, X-ray crystallography and nuclear magnetic resonance (NMR). Three dimensional transmission electron microscopy (3DTEM) is one of the most sophisticated techniques for structure determination of molecular machines. Known to give the 3-dimensional structures in its native form with literally no upper limit on size of the macromolecule, this tool does not need the crystallization of the protein. Combining the 3DTEM data with in silico tools, one can have better refined structure of a desired complex. In this review we are discussing about the recent advancements in three dimensional electron microscopy and tools associated with it.
In text/plain
format
Archived Files and Locations
application/pdf 190.8 kB
file_qldqhfgn7ndbhijxsy7tumvypm
|
europepmc.org (repository) web.archive.org (webarchive) |
access all versions, variants, and formats of this works (eg, pre-prints)