Three dimensional electron microscopy and in silico tools for macromolecular structure determination release_sny5lwvfnbckhpk3n2anh7dx7q

by Subhomoi Borkotoky, Chetan Kumar Meena, Mohammad Wahab Khan, Ayaluru Murali

Published in EXCLI Journal : Experimental and Clinical Sciences.

2013   Volume 12, p335-46

Abstract

Recently, structural biology witnessed a major tool - electron microscopy - in solving the structures of macromolecules in addition to the conventional techniques, X-ray crystallography and nuclear magnetic resonance (NMR). Three dimensional transmission electron microscopy (3DTEM) is one of the most sophisticated techniques for structure determination of molecular machines. Known to give the 3-dimensional structures in its native form with literally no upper limit on size of the macromolecule, this tool does not need the crystallization of the protein. Combining the 3DTEM data with in silico tools, one can have better refined structure of a desired complex. In this review we are discussing about the recent advancements in three dimensional electron microscopy and tools associated with it.
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Type  article-journal
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Date   2013-04-24
Language   en ?
PubMed  27092033
PMC  PMC4827587
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ISSN-L:  1611-2156
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