Chitosan nanoparticles as a new technique in gene transformation into different plants tissues release_rxesgib7dneufgjd5e3m7acse4

by Eman Tawfik, Mohamed Fathy

Published in Natural Resources for Human Health by Visagaa Publishing House.



The utilization of chitosan nanoparticles is a novel technique for gene transformation into plant tissues. It takes a few minutes to transform gene to plant. UidA gene was detected in <i>Escherichia coli</i> (K12 strain) using polymerase chain reaction analysis by UidA-specific primers. The gene was transformed into the explants of two different plant species (<i>Solanum tuberosum</i> and <i>Paulownia tomentosa</i>). These plants have different natures as crop and woody plants respectively. Therefore, they have different abilities to express the UidA gene. The gene is expressed into blue color in plant tissues due to the formation and expression of the GUS enzyme. The transformation of the UidA gene was detected morphologically by the formation of blue color; and molecular using PCR. Chitosan nanoparticles were characterized by UV/Visible spectroscope and photographing with a transmission electron microscope (TEM). As a result of this research, it is suggested that chitosan nanoparticles be used in gene transformation into plant tissues. Because it is safe, quick, and inexpensive, as well as biocompatible and biodegradable.
In application/xml+jats format

Archived Files and Locations

application/pdf  1.1 MB
file_75zk3s3i2fcerogy4on7pcbsre (publisher) (webarchive)
Read Archived PDF
Preserved and Accessible
Type  article-journal
Stage   published
Date   2022-01-07
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: bd3b4f01-6825-4471-9083-f9b9ab225538