Reduced graphene oxide–based field effect transistors for the detection of E7 protein of human papillomavirus in saliva release_riok3izpmrgvbpdpl77nqff234

by Patrik Aspermair, Vladyslav Mishyn, Johannes Bintinger, Henri Happy, Komal Bagga, Palaniappan Subramanian, Wolfgang Knoll, Rabah Boukherroub, Sabine Szunerits

Published in Analytical and Bioanalytical Chemistry by Springer Science and Business Media LLC.

2020  

Abstract

Several challenging biological sensing concepts have been realized using electrolyte-gated reduced graphene oxide field effect transistors (rGO-FETs). In this work, we demonstrate the interest of rGO-FET for the sensing of human papillomavirus (HPV), one of the most common sexually transmitted viruses and a necessary factor for cervical carcinogenesis. The highly sensitive and selective detection of the HPV-16 E7 protein relies on the attractive semiconducting characteristics of pyrene-modified rGO functionalized with RNA aptamer Sc5-c3. The aptamer-functionalized rGO-FET allows for monitoring the aptamer-HPV-16 E7 protein binding in real time with a detection limit of about 100 pg mL-1 (1.75 nM) for HPV-16 E7 from five blank noise signals (95% confidence level). The feasibility of this method for clinical application in point-of-care technology is evaluated using HPV-16 E7 protein suspended in saliva and demonstrates the successful fabrication of a promising field effect transistor biosensor for HPV diagnosis.Graphical abstract.
In text/plain format

Archived Files and Locations

application/pdf  1.5 MB
file_hzxkpkqn5jad5guf7zejwoyzxy
link.springer.com (publisher)
web.archive.org (webarchive)
Read Archived PDF
Preserved and Accessible
Type  article-journal
Stage   published
Date   2020-08-20
Language   en ?
Container Metadata
Not in DOAJ
In Keepers Registry
ISSN-L:  1618-2642
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: 4393795a-bb6b-4cb8-9167-c93faee51418
API URL: JSON