The Effects of Different Concentrations and Combinations of Growth Regulators on the Micro propagation Of Potato (Solanum Tubrosum)
Bhawna Chaudhary, Pallavi Mittal
In this investigation, a high frequency single step and a simple method of direct plant regeneration has been developed for two potato cultivars K.CH3(Kufri. CHIPSONA 3) and K.Jyoti. (Kufri Jyoti) iinternodes were excised from field grown plants and cultured on MS media supplemented with different concentrations and combinations of auxins (NAA and IAA), cytokinins (BAP and Zeatin) and GA3. This study was aimed to evaluate the effect of different growth regulators (GRs) in seven different combinations named as T1, T2, T3, T4, T5, T6 and T7 along with control T0 (no growth regulators) on mass propagation of two potato cultivars K.CH3 and K.Jyoti. The best regeneration of internodes was obtained when MS medium was supplemented with 1.0 mgL-1 GA3, 0.01 mgL-1 IAA and 2.0 mgL-1 Zeatin (T2). This combination took minimum time for regeneration of multiple shoots and roots on internodes of cultivar K.CH3. Further, with either decrease, increase or change the combination of growth regulators from the optimum level, a significant decline in percent plant regeneration as well as number of shoots per explant was recorded.
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