Discovery of a Pseudomonas aeruginosa Type VI secretion system toxin targeting bacterial protein synthesis using a global genomics approach release_rev_48cfcf70-f2d5-4854-88a1-08d7dc83bb8d

by Laura M Nolan, Amy K Cain, Eleni Manoli, Maria A Sainz-Polo, Gordon Dougan, Despoina A. I Mavridou, David Albesa-Jove, Julian Parkhill, Alain Filloux


The Type VI secretion system (T6SS) is a bacterial weapon which delivers toxic effectors to kill competitors or subvert some of their key functions. Here we use transposon directed insertion-site sequencing (TraDIS) to identify T6SS toxins associated with the H1-T6SS, one of the three T6SS machines found in Pseudomonas aeruginosa. This approach identified several putative toxin-immunity pairs, including Tse8-Tsi8. Full characterization of this protein pair demonstrated that Tse8 is delivered by the VgrG1a spike complex into prey cells where it targets the transamidosome, a multiprotein complex involved in protein synthesis in bacteria lacking either one or both of the asparagine or glutamine tRNA synthases. Our data suggests that Tse8 combines as a non-cognate component of the transamidosome complex, reducing fitness by limiting the ability of the cell to synthesize proteins. This is the first demonstration of a T6SS toxin affecting protein synthesis, expanding the range of cellular components targeted by this bacterial weapon. The success of the current study validates the use of our TraDIS approach as a tool to drastically expand the repertoire of T6SS toxins in any T6SS-encoding bacterium.
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Released as a post by Cold Spring Harbor Laboratory
Release Date 2019-08-13
Publisher Cold Spring Harbor Laboratory
Type  post
Stage   unknown
Date   2019-08-13
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