Forensic microbial system for high-resolution object provenance
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by
Jason Qian, Zhixiang Lu, Christopher Mancuso, Han-Ying Jhuang, Rocío Barajas Ornelas, Sarah Boswell, Fernando H Ramírez-Guadiana, Victoria Jones, Akhila Sonti, Kole Sedlack, Lior Artzi, Giyoung Jung (+9 others)
2020
Abstract
Mapping where an object has been is a fundamental challenge for human health, commerce, and food safety. Location-specific microbes offers the potential to cheaply and sensitively determine object provenance. We created a synthetic, scalable microbial spore system that identifies object provenance in under one hour at meter-scale resolution and near single spore sensitivity, and that can be safely introduced into and recovered from the environment. This system solves the key challenges in object provenance: persistence in the environment, scalability, rapid and facile decoding, and biocontainment. Our system is compatible with SHERLOCK, facilitating its implementation in a wide range of applications.
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Date 2020-03-16
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