EFFECTS OF THE VIRULENT BACTERIOPHAGES ON KLEBSIELLA PNEUMONIAE BIOFILMS
ВОЗДЕЙСТВИЕ ВИРУЛЕНТНЫМИ БАКТЕРИОФАГАМИ НА БИОПЛЕНКИ KLEBSIELLA PNEUMONIAE release_qjilz24wsnhkzkvqu7pe33whr4

by E.A. Glazunov, Research and production center «MicroMir», Moscow 107031, Russian Federation, F.M. Zurabov, I.B. Pavlova, G.S. Tolmacheva, Lomonosov Moscow State University, Moscow 119234, Russian Federation, All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of Federal State Budget Scientific Institution «Federal Scientific Center – K. I. Skryabin, Ya. R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences», Moscow 123022, Russian Federation, All-Russian Research Institute of Veterinary Sanitation, Hygiene and Ecology – Branch of Federal State Budget Scientific Institution «Federal Scientific Center – K. I. Skryabin, Ya. R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences», Moscow 123022, Russian Federation

Published in Problems of Veterinary Sanitation, Hygiene and Ecology by The publishing house - SCIENTIFIC LIВRARY.

p480-485 (2020)

Abstract

One of the most important problems of our time is the growing level of resistance to antibiotics among human and animal pathogens. In this regard, more and more attention is paying to alternative therapeutic agents. In the present work, the effects of the bacteriophage vB_KpnP_FZ12 on Klebsiella pneumoniae biofilms have been investigated. Klebsiella pneumoniae 315 strain and the bacteriophage vB_KpnP_FZ12 were used. To study the formation of biofilms in liquid nutrient media by populations of Klebsiella pneumoniae 315, 17…18-hour cultures of microorganisms in the Sform (third generation) were used. A suspension of bacteria at a concentration of 1×105 CFU/ml in a volume of 5 ml was mixed on machine Vortex and introduced into Petri dishes with 20 ml of BHI broth. Sterile slides were placed on the bottom of a Petri dish, sterile fat-free cover glasses were placed on top, and incubated in a thermostat for 24-48 hours at 37°C. After 24 hours, 0.1 ml of the bacteriophage vB_KpnP_FZ12 was added with a concentration of 1×106 PFU/ml, incubated in a thermostat for 24 hours at a temperature of 37°C. In parallel, control glasses were incubated without adding phage. To preserve the natural architectonics, the samples were fixed in vivo in vapors of 25% glutaraldehyde for 3...5 h. It was shown that the bacteriophage is able to effectively destroy the structure of biofilms and clusters, while reducing the total number of bacteria.
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