Identification and functional analysis of cation-efflux transporter 1 from Brassica juncea L.
release_pks2gue7gbgcfi6rumz3qt3jja
by
Lu Han, Xiaohua Wu, Xinyu Zhang, Kailin Hou, Hongshan Zhang, Chenjia Shen
2022 Volume 22, Issue 1, p174
Abstract
<jats:title>Abstract</jats:title><jats:sec>
<jats:title>Background</jats:title>
<jats:italic>Brassica juncea</jats:italic> behaves as a moderate-level accumulator of various heavy metal ions and is frequently used for remediation. To investigate the roles of metal ion transporters in <jats:italic>B. juncea</jats:italic>, a cation-efflux family gene, <jats:italic>BjCET1</jats:italic>, was cloned and functionally characterized.
</jats:sec><jats:sec>
<jats:title>Results</jats:title>
BjCET1 contains 382 amino acid residues, including a signature motif of the cation diffusion facilitator protein family, six classic trans-membrane-spanning structures and a cation-efflux domain. A phylogenetic analysis showed that BjCET1 has a high similarity level with metal tolerance proteins from other <jats:italic>Brassica</jats:italic> plants, indicating that this protein family is highly conserved in <jats:italic>Brassica</jats:italic>. <jats:italic>BjCET1</jats:italic> expression significantly increased at very early stages during both cadmium and zinc treatments. Green fluorescence detection in transgenic tobacco leaves revealed that BjCET1 is a plasma membrane-localized protein. The heterologous expression of BjCET1 in a yeast mutant increased the heavy-metal tolerance and decreased the cadmium or zinc accumulations in yeast cells, suggesting that BjCET1 is a metal ion transporter. The constitutive expression of BjCET1 rescued the heavy-metal tolerance capability of transgenic tobacco plants.
</jats:sec><jats:sec>
<jats:title>Conclusions</jats:title>
The data suggest that BjCET1 is a membrane-localized efflux transporter that plays essential roles in heavy metal ion homeostasis and hyper-accumulation.
</jats:sec>
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