Identification and functional analysis of cation-efflux transporter 1 from Brassica juncea L. release_pks2gue7gbgcfi6rumz3qt3jja

by Lu Han, Xiaohua Wu, Xinyu Zhang, Kailin Hou, Hongshan Zhang, Chenjia Shen

Published in BMC Plant Biology by Springer Science and Business Media LLC.

2022   Volume 22, Issue 1, p174

Abstract

<jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:italic>Brassica juncea</jats:italic> behaves as a moderate-level accumulator of various heavy metal ions and is frequently used for remediation. To investigate the roles of metal ion transporters in <jats:italic>B. juncea</jats:italic>, a cation-efflux family gene, <jats:italic>BjCET1</jats:italic>, was cloned and functionally characterized. </jats:sec><jats:sec> <jats:title>Results</jats:title> BjCET1 contains 382 amino acid residues, including a signature motif of the cation diffusion facilitator protein family, six classic trans-membrane-spanning structures and a cation-efflux domain. A phylogenetic analysis showed that BjCET1 has a high similarity level with metal tolerance proteins from other <jats:italic>Brassica</jats:italic> plants, indicating that this protein family is highly conserved in <jats:italic>Brassica</jats:italic>. <jats:italic>BjCET1</jats:italic> expression significantly increased at very early stages during both cadmium and zinc treatments. Green fluorescence detection in transgenic tobacco leaves revealed that BjCET1 is a plasma membrane-localized protein. The heterologous expression of BjCET1 in a yeast mutant increased the heavy-metal tolerance and decreased the cadmium or zinc accumulations in yeast cells, suggesting that BjCET1 is a metal ion transporter. The constitutive expression of BjCET1 rescued the heavy-metal tolerance capability of transgenic tobacco plants. </jats:sec><jats:sec> <jats:title>Conclusions</jats:title> The data suggest that BjCET1 is a membrane-localized efflux transporter that plays essential roles in heavy metal ion homeostasis and hyper-accumulation. </jats:sec>
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