Free-running enzymatic oligonucleotide synthesis for data storage applications release_omynutjes5gqrgshizkng7lt3m

by Michael Jensen, Peter Griffin, Ronald W Davis

Released as a post by Cold Spring Harbor Laboratory.

2018  

Abstract

Here we present preliminary results for a method of oligodeoxynucleotide synthesis using terminal deoxynucleotidyl transferase (TdT) to generate mixed base homopolymer runs of defined sequence. In a process we have termed Free-Running Synthesis (FRS), we allow TdT to freely add multiple bases of a given monomer. With this method, homopolymeric runs can be used for writing DNA in a data storage capacity, where a stretch of As, for example, will be read as a single base, and the transitions between successive homopolymer runs encodes information. As a proof-of-concept, we demonstrate homopolymer additions of A, C and T onto the 3 prime end of a 22 base initiator strand.
In application/xml+jats format

Archived Files and Locations

application/pdf  3.3 MB
file_ixozsl5tgjevlinvdmu256tr3y
www.biorxiv.org (repository)
web.archive.org (webarchive)
application/pdf  3.3 MB
file_vcmepddeebbzzn7m4e554n7wui
web.archive.org (webarchive)
www.biorxiv.org (web)
Read Archived PDF
Preserved and Accessible
Type  post
Stage   unknown
Date   2018-06-30
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: 392981c4-ddae-4f47-a996-706ed697f9c1
API URL: JSON