Flexible Label-Free Platinum and Bio-PET-Based Immunosensor for the Detection of SARS-CoV-2 release_o5po7s5i2vhxdd32bzpdtrfpae

by Rodrigo Vieira Blasques, Paulo Roberto de Oliveira, Cristiane Kalinke, Laís Canniatti Brazaca, Robert Crapnell, Juliano Alves Bonacin, Craig E. Banks, Bruno Janegitz

Published in Biosensors by MDPI AG.

2023   Volume 13, Issue 2, p190


The demand for new devices that enable the detection of severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) at a relatively low cost and that are fast and feasible to be used as point-of-care is required overtime on a large scale. In this sense, the use of sustainable materials, for example, the bio-based poly (ethylene terephthalate) (Bio-PET) can be an alternative to current standard diagnostics. In this work, we present a flexible disposable printed electrode based on a platinum thin film on Bio-PET as a substrate for the development of a sensor and immunosensor for the monitoring of COVID-19 biomarkers, by the detection of L-cysteine and the SARS-CoV-2 spike protein, respectively. The electrode was applied in conjunction with 3D printing technology to generate a portable and easy-to-analyze device with a low sample volume. For the L-cysteine determination, chronoamperometry was used, which achieved two linear dynamic ranges (LDR) of 3.98−39.0 μmol L−1 and 39.0−145 μmol L−1, and a limit of detection (LOD) of 0.70 μmol L−1. The detection of the SARS-CoV-2 spike protein was achieved by both square wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS) by a label-free immunosensor, using potassium ferro-ferricyanide solution as the electrochemical probe. An LDR of 0.70−7.0 and 1.0−30 pmol L−1, with an LOD of 0.70 and 1.0 pmol L−1 were obtained by SWV and EIS, respectively. As a proof of concept, the immunosensor was successfully applied for the detection of the SARS-CoV-2 spike protein in enriched synthetic saliva samples, which demonstrates the potential of using the proposed sensor as an alternative platform for the diagnosis of COVID-19 in the future.
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