Original Article Effects of aconitine on the proliferation and apoptosis of human cholangiocarcinoma cells release_nvp4hldwrjeznif42fvsa3e3iy

Abstract

Objectives: The aim of the study was to investigate the effect of aconitine on the proliferation and apop-tosis of human cholangiocarcinoma QBC-939 cells and to explore its possible mechanism. Method: Human chol-angiocarcinoma QBC-939 cells were treated with various concentrations of aconitine. Cell viability was detected by the CCK8 assay; Cell apoptosis was evaluated by flow cytometry analysis. The expression levels of stathmin and cyclinD1 were examined by quantitive PCR and western blotting. Results: We found that aconitine could significantly inhibited the proliferation of QBC-939 cells in a dose-dependent manner, meanwhile, the apoptotic rates of QBC-939 cells were also significantly enhanced by aconitine treatment with increasing concentration. Additonally aconi-tine made the cell cycle shift to a high G1 phase, especially at high dose. The quantitative PCR results showed that aconitine decreased the expressions of stathmin and cyclinD1 at mRNA level. The following Western blot analysis came to similar conclusion to PCR at protein level in aconitine treated cells. Conclusion: Aconitine could significantly inhibit cell proliferation and induce cell apoptosis of human cholangiocarcinoma cell line QBC-939 and the mechanism may be related to cell cycle arresting at G1 phase.
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