Activity of Medicinal Plant Extracts on Multiplication ofMycobacterium tuberculosisunder Reduced Oxygen Conditions Using Intracellular and Axenic Assays release_nt4a3mewpnafphvshnfjd52rnq

by Purva D. Bhatter, Pooja D. Gupta, Tannaz J. Birdi

Published in International Journal of Microbiology by Hindawi Limited.

2016   Volume 2016, p1-6


<jats:italic>Aim.</jats:italic>Test the activity of selected medicinal plant extracts on multiplication of<jats:italic>Mycobacterium tuberculosis</jats:italic>under reduced oxygen concentration which represents nonreplicating conditions.<jats:italic>Material and Methods.</jats:italic>Acetone, ethanol and aqueous extracts of the plants<jats:italic>Acorus calamus</jats:italic>L. (rhizome),<jats:italic>Ocimum sanctum</jats:italic>L. (leaf),<jats:italic>Piper nigrum</jats:italic>L. (seed), and<jats:italic>Pueraria tuberosa</jats:italic>DC. (tuber) were tested on<jats:italic>Mycobacterium tuberculosis</jats:italic>H37Rv intracellularly using an epithelial cell (A549) infection model. The extracts found to be active intracellularly were further studied axenically under reducing oxygen concentrations.<jats:italic>Results and Conclusions.</jats:italic>Intracellular multiplication was inhibited ≥60% by five of the twelve extracts. Amongst these 5 extracts, in axenic culture,<jats:italic>P. nigrum</jats:italic>(acetone) was active under aerobic, microaerophilic, and anaerobic conditions indicating presence of multiple components acting at different levels and<jats:italic>P. tuberosa</jats:italic>(aqueous) showed bactericidal activity under microaerophilic and anaerobic conditions implying the influence of anaerobiosis on its efficacy.<jats:italic>P. nigrum</jats:italic>(aqueous) and<jats:italic>A. calamus</jats:italic>(aqueous and ethanol) extracts were not active under axenic conditions but only inhibited intracellular growth of<jats:italic>Mycobacterium tuberculosis</jats:italic>, suggesting activation of host defense mechanisms to mediate bacterial killing rather than direct bactericidal activity.
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Type  article-journal
Stage   published
Year   2016
Language   en ?
DOI  10.1155/2016/8073079
PubMed  26941797
PMC  PMC4752996
Wikidata  Q36578384
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ISSN-L:  1687-918X
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