@misc{guo_mao_guo_an_zhang_song_wang_duan_zhang_yang_et al._2021, 
  title={Alternatively Spliced ANLN Isoforms Synergistically Contribute To The Progression of Head and Neck Squamous Cell Carcinoma}, 
  DOI={10.21203/rs.3.rs-375675/v1}, 
  abstractNote={<jats:title>Abstract</jats:title>
        <jats:p><jats:bold>Background:</jats:bold> Head and neck squamous cell carcinoma (HNSCC) is a common cancer with high mortality. Anilin actin binding protein (ANLN) has been reported to be associated with carcinogenesis in multiple tumors. However, the expression pattern and functional effects of ANLN in HNSCC remains to be unclear.<jats:bold>Methods: </jats:bold>Expression of two major splice variants of ANLN was assessed in HNSCC tissues and cell lines. The functional effects and related mechanisms of ANLN isoforms were investigated in HNSCC <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic>.<jats:bold>Results: </jats:bold>Our results showed that the two primary isoforms of ANLN transcripts ANLN-201 and ANLN-210 were highly expressed in HNSCC tissues and cell lines. Knockout of ANLN restrained cell proliferation, migration and invasion of SCC-9 cells. Mechanically, ANLN-201 could interact with c-Myc to keep its protein stability, thereby playing a oncogenic role in HNSCC. ANLN-210 could be transferred to macrophages via exosomes by binding to RNA binding protein hnRNPC. Exosomal ANLN-210 promoted macrophage polarization via PTEN/PI3K/Akt signaling pathway, thus stimulating tumor growth of HNSCC.<jats:bold>Conclusions: </jats:bold>Our findings demonstrate that alternatively spliced ANLN isoform<jats:bold>s</jats:bold> collaboratively promote HNSCC tumorigenesis <jats:italic>in vitro</jats:italic> and <jats:italic>in vivo</jats:italic>, which might provide the in-depth role and mechanism of ANLN in HNSCC development.</jats:p>}, 
  publisher={Research Square}, 
  author={Guo, Erliang and Mao, Xionghui and Guo, Lunhua and An, Changming and Zhang, Cong and Song, Kaibin and Wang, Guohui and Duan, Chunbin and Zhang, Xiwei and Yang, Xianguang and et al.}, 
  year={2021}, 
  month={Apr}
  }