The effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats release_mqmhbovzfbbkvpx5vtnof4fc5q

by Eviana Budiartanti Sutanto, Taufiq R Nasihun, Israhnanto Isradji, Luciana Budiati Sutanto

Published in World Nutrition Journal by Indonesian Nutrition Association.

2017   p28

Abstract

Introduction: Cigarette smoke causes oxidative stress which results in reduced sperm concentration, motility and morphology, also increased levels of 8-OHdG as a marker of DNA damage. Vitamin C and E have potential role in repairing spermatozoa damages. The aim of this study was to determine the effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats.Methods: This study used a post test only control group design among 18 male Wistar rats subject, aged 8 week, 150-200 grams body weight (BW). The subject was randomly divided into 3 groups, K1: control, K2: cigarettes smoke exposed, K3: cigarettes smoke exposed and given a combination of 0.045 mg/gBW vitamin C and 0.036 IU/gBW vitamin E per oral. Analysis was done on day 21 using one-way ANOVA and post-hoc LSD for sperm concentration, motility and morphology; using Kruskal-Wallis and Mann-Whitney tests for cement 8- OHdG levels.Results: The lowest sperm concentration was found in   K2 (K2  32.59  million/mL,  K1 47.91 million/mL, K 339.43 million/mL); the lowest normal sperm motility was found in K2 (K 238.97%, K 164.57%, K3 51.43%); the lowest normal sperm morphology was found in K2 (K2 27.56%, K 138.36%, K 331.18%); and the highest cement 8- OHdG level was found in K2 (K2 20.18ng/mL, K1 3.43ng/mL, K3 5.28ng/mL).Conclusion: Combination of vitamin C and E can improve sperm concentration, motility and morphology and decrease cement 8-OHdG levels of smoke exposed rats.
In application/xml+jats format

Archived Files and Locations

application/pdf  309.5 kB
file_65q3cvcvxrdxnnjzokpudotsj4
worldnutrijournal.org (web)
web.archive.org (webarchive)
Read Archived PDF
Preserved and Accessible
Type  article-journal
Stage   published
Date   2017-08-15
Container Metadata
Open Access Publication
In DOAJ
In Keepers Registry
ISSN-L:  2580-7013
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: b29917f7-24fe-4e3f-a823-3ceba4258e3f
API URL: JSON