Tachykinin stimulation effects on α-amylase, protease and lipase activities in midgut of American cockroach, Periplaneta americana (Blattodea: Blattidae)
release_kh5sbe6m5zfehgmjip5wcf37zy
by
A Mikani
2016 Volume 36
Abstract
The effect of a neuropeptide, the tachykinin on α-amylase, protease and lipase activities in the midgut of American cockroach, Periplaneta americana (Linnaeus) is evaluated. Immunohistochemical reactivities against tachykinin (tachykinin-ir) were detected in the midgut. Tachykinin-ir cells in three regions (anterior, median and posterior) of the midgut were counted in normally fed, starved for four weeks and refed cockroaches. The number of tachykinin-ir cells decreased during 4 weeks of starvation and increased 3 h after refeeding. The results of the competitive ELISA were consistent with the profile of immunohistochemical reactivity. Incubation of the dissected midgut with tachykinin led to an increase in α-amylase, protease and lipase activities while the buffer remained ineffective. Tachykinin injection into the hemocoel increased α-amylase, protease and lipase activities, but PBS injection showed no sign of effectiveness. The results seggest that nutrients upregulate midgut cells secreting tachykinin causing further activities of digestive enzymes. ﭼﮑﯿﺪه ﺗﺎﮐﯿﮑ ﯿ ﻨ ﯿﻦ : آﻧﺰﯾﻢ ﻓﻌﺎﻟﯿﺖ ﮐﻨﻨﺪه ﺗﺤﺮﯾﮏ آﻣﺮﯾﮑﺎﯾﯽ ﺳﻮﺳﺮي ﻣﯿﺎﻧﯽ ﻣﻌﺪه در ﻟﯿﭙﺎز و ﭘﺮوﺗﺌﺎز ،آﻣﯿﻼز آﻟﻔﺎ ﻫﺎي Periplaneta americana (Blattodea: Blattidae) اﻋﻈﻢ ﻣﯿﮑﺎﻧﯽ ،آﻣﯿﻼز آﻟﻔﺎ ﻓﻌﺎﻟﯿﺖ روي ﺑﺮ ﺗﺎﮐﯿﮑﯿﻨﯿﻦ اﺛﺮ ﺑﺮرﺳﯽ ﺑﻪ ﺗﺤﻘﯿﻖ اﯾﻦ در ﻣ ﻣﻌﺪه در ﻟﯿﭙﺎز و ﭘﺮوﺗﺌﺎز اﻣﺮﯾﮑﺎﯾﯽ ﺳﻮﺳﺮي ﯿﺎﻧﯽ) L. (Periplaneta americana ﭘﺮداﺧﺘﻪ ﺷﺪ. ﺳﻠﻮل اﯾﻤﻨﻮﻫﯿﺴﺘﻮﺷﯿﻤﯿﺎﯾﯽ ﻓﻌﺎﻟﯿﺖ ﻣﯿﺎﻧﯽ ﻣﻌﺪه ﻫﺎي) ﻋﻘﺒﯽ و ﻣﯿﺎﻧﯽ ،ﺟﻠﻮﯾﯽ ﻫﺎي ﺑﺨﺶ (ﮐﻪ ﻣﯽ ﺗﺮﺷﺢ ﺗﺎﮐﯿﮑﯿﻨﯿﻦ ﮔﺮﻓﺖ ﻗﺮار ﺗﺎﯾﯿﺪ ﻣﻮرد ﮐﺮدﻧﺪ. ﻣﻘﺎﺑﻞ اﯾﻤﻨﻮﻫﯿﺴﺘﻮﺷﯿﻤﯿﺎﯾﯽ اﻟﻌﻤﻞ ﻋﮑﺲ ﮐﻪ را ﻫﺎﯾﯽ ﺳﻠﻮل ﺗﻌﺪاد ،ﮔﺮﺳﻨﮕﯽ ﻫﻔﺘﻪ ﭼﻬﺎر آ ﻧﺘﯽ داﺷﺘﻨﺪ ﺗﺎﮐﯿﮑﯿﻨﯿﻦ ﺑﺎدي) ir cells-Tachykinin (ﺳﺎﻋﺖ ﺳﻪ از ﭘﺲ دوﺑﺎره ﺗﻐﺬﯾﻪ ﮐﻪ ﺣﺎﻟﯽ در ،داد ﮐﺎﻫﺶ را ir cells-Tachykinin را داد اﻓﺰاﯾﺶ. ﮐﺮد ﺗﺎﯾﯿﺪ را ﻓﻮق ﻧﺘﯿﺠﻪ ﻧﯿﺰ اﻻﯾﺰا آزﻣﻮن. ﻓﻌﺎﻟﯿﺖ اﻓﺰاﯾﺶ ﺑﻪ ﻣﻨﺠﺮ ﺗﺎﮐﯿﮑﯿﻨﯿﻦ ﺣﺎوي ﺑﺎﻓﺮ در ﻣﯿﺎﻧﯽ ﻣﻌﺪه دادن ﻗﺮار ،آﻣﯿﻼز آﻟﻔﺎ ﺗﻨﻬﺎ ﺑﻪ ﺑﺎﻓﺮ ﮐﻪ ﺣﺎﻟﯽ در ﺷﺪ ﻟﯿﭙﺎز و ﭘﺮوﺗﺌﺎز آﻧﺰﯾﻢ ﻓﻌﺎﻟﯿﺖ روي ﺑﺮ اﺛﺮي ﻫﯿﭻ ﯾﯽ ﻧﺪاﺷﺖ ﻓﻮق ﻫﺎي. ﺣ ﻫﻤﻮﻟﻨﻒ ﺑﻪ ﺗﺎﮐﯿﮑﯿﻨﯿﻦ ﺗﺰرﯾﻖ ﺑﻪ ﻣﻨﺠﺮ ﺸﺮه آﻧﺰﯾﻢ ﻓﻌﺎﻟﯿﺖ اﻓﺰاﯾﺶ در ﻓﻮق ﻫﺎي ﺑﺮ ﺗﺎﺛﯿﺮي ﻫﯿﭻ ﺑﺎﻓﺮ ﺗﺰرﯾﻖ ﮐﻪ ﺣﺎﻟﯽ در ،ﺷﺪ ﻣﯿﺎﻧﯽ ﻣﻌﺪه ﻓﻌﺎﻟﯿ روي ﻧﺪاﺷﺖ ﻫﺎ آﻧﺰﯾﻢ ﺖ. ﮐﻠﯽ ﻃﻮر ﺑﻪ ﻣﯽ ﺗﻮان ﻫﺎي آﻧﺰﯾﻢ ﻓﻌﺎﻟﯿﺖ ﺧﻮد ﻧﻮﺑﻪ ﺑﻪ ﻧﯿﺰ آن ﮐﻪ ﺷﺪه ﻣﯿﺎﻧﯽ ﻣﻌﺪه ﻫﺎي ﺳﻠﻮل از ﺗﺎﮐﯿﮑﯿﻨﯿﻦ ﺗﺮﺷﺢ ﺑﺎﻋﺚ ﺗﻐﺬﯾﻪ ﮐﻪ ﮔﺮﻓﺖ ﻧﺘﯿﺠﻪ ،آﻣﯿﻼز آﻟﻔﺎ ﻣﯽ اﻓﺰاﯾﺶ را ﻟﯿﭙﺎز و ﭘﺮوﺗﺌﺎز دﻫﺪ .
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