Service is in read-only mode for maintenance. See gitter chat for updates.

Geographic discrimination of Paracoccidioides brasiliensis strains by randomly amplified polymorphic DNA analysis release_itusrcskd5a6nea2g2a65l7jom

by A M Calcagno, G Niño-Vega, F San-Blas, G San-Blas

Published in Journal of Clinical Microbiology.

1998   Volume 36, Issue 6, p1733-6


Randomly amplified polymorphic DNA (RAPD) analysis of 33 Paracoccidioides brasiliensis strains from Argentina, Brazil, Colombia, Peru, and Venezuela produced reproducible amplification products which were sufficiently polymorphic to allow differentiation of the strains. Types generated with five primers (OPG 03, OPG 05, OPG 14, OPG 16, and OPG 18) resulted in a high discriminatory index (0.956). The discriminatory index was slightly reduced (0.940) when only two primers (OPG 3 and OPG 14) were used. A dendrogram based on these results showed a high degree of similarity among the strains, and genetic differences were expressed in clusters related to geographical regions but not to pathological features of the disease. With a few exceptions, strains were sorted into five groups by geographical origin as follows: group I, Venezuelan strains; group II, Brazilian strains; group III, Peruvian strains; group IV, Colombian strains; and group V, Argentinian strains. The group containing the most disparate strains was group V (discriminatory index, 0.633); the discriminatory index for the other four groups was 0.824. The use of primer OPG 18 by itself was sufficient to discriminate species specificity, and the use of primer OPG 14 by itself was sufficient to discriminate among the geographical locations of the strains in the sample. This method may be helpful for epidemiological studies of P. brasiliensis.
In text/plain format

Archived Files and Locations

application/pdf  256.0 kB
file_lc2wla2d6remvaalvqibntboy4 (web) (webarchive)
application/pdf  253.0 kB
file_4gu7kcbctjdchhob64k3etogua (repository) (webarchive)
Read Archived PDF
Type  article-journal
Stage   published
Year   1998
Language   en ?
PubMed  9620409
PMC  PMC104909
Journal Metadata
Not in DOAJ
In Keepers Registery
ISSN-L:  0095-1137
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: 4beb5870-fc1b-4df4-9b31-bd2495b7a30b