Characterization and Identification of a Ripening-Related Gene AaPG18 in Actinidia arguta release_hdh6srknz5ey5drhj3vzlvk3hm

by Yukuo Li, Hailei Huang, Muhammad Abid, Hong Gu, Jinbao Fang, Zhongping Cheng, Xiujuan Qi

Published in International Journal of Molecular Sciences by MDPI AG.

2022   Volume 23, Issue 5, p2597

Abstract

Actinidia arguta (A. arguta) is a kind of climacteric fruit that quickly softens and limits fruit shelf-life and commercial value. Therefore, it is of great significance to develop kiwifruit genotypes with an extended shelf-life of fruit. However, the ripening and softening mechanisms remain unclear in A. arguta. Here, we demonstrated that a key polygalacturonase (PG)-encoding gene AaPG18 was involved in A. arguta ripening through the degradation of the cell wall. Fruits were harvested at three developmental stages (S1, S2, and S3) for high-throughput transcriptome sequencing, based on which two candidate transcripts c109562_g1 and c111961_g1 were screened. The genome-wide identification of the PG gene family assigned c109562_g1 and c111961_g1 to correspond to AaPG4 and AaPG18, respectively. The expression profiles of candidate genes at six preharvest stages of fruit showed significantly higher expression levels of AaPG18 than AaPG4, indicating AaPG18 might be a key gene during fruit ripening processes. The subcellular localization displayed AaPG18 was located at the cytoplasmic membrane. The transient overexpression of AaPG18 in strawberry and the following morphological observation suggested AaPG18 played a key role in maintaining the stability of cell morphology. The homologous transient transformation in A. arguta "RB-4" proved the crucial function of AaPG18 in fruit ripening processes by causing the rapid redness of the fruit, which was an indicator of fruit maturity. All in all, our results identified AaPG18 as a key candidate gene involved in cell wall degeneration, which provides a basis for the subsequent exploration of the molecular mechanisms underlying the ripening and softening of A. arguta fruit.
In application/xml+jats format

Archived Files and Locations

application/pdf  4.9 MB
file_tyz7qcajkzacjawehrfhxlqf4e
mdpi-res.com (publisher)
web.archive.org (webarchive)

Web Captures

https://www.mdpi.com/1422-0067/23/5/2597/htm
2022-05-02 13:36:16 | 45 resources
webcapture_cj4u6ajjbvauvm2geaixua3ely
web.archive.org (webarchive)
Read Archived PDF
Preserved and Accessible
Type  article-journal
Stage   published
Date   2022-02-26
Language   en ?
Journal Metadata
Open Access Publication
In DOAJ
In ISSN ROAD
In Keepers Registry
ISSN-L:  1422-0067
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: 65964be3-8775-422f-886e-34aa57a562a5
API URL: JSON