Interactions of Secondary DNA and Initial DNA on Single-Walled Carbon Nanotube Surfaces Studied by Photoluminescence, Atomic Force Microscopy, and Electrophoresis release_gy7ne4drmjgzncz7eblf3uq6fi

by Reona Toyofuku, Shusuke Ohura, Masahiro Ito, Yoshikazu Homma, Kazuo Umemura

Published in Journal of Nanomaterials by Hindawi Limited.

2019   Volume 2019, p1-8

Abstract

We examined the interactions of initial single-stranded DNA (ssDNA) and secondary ssDNA molecules on single-walled carbon nanotubes (SWNTs). Thymine 30-mers (T30) and 30-mers from a partial sequence of <jats:italic>φ</jats:italic>x174 DNA (<jats:italic>φ</jats:italic>30) were used to prepare the DNA-SWNT hybrids. First, the hybrids were annealed at various temperatures without secondary DNA to evaluate the stability of the hybrids. As a result, aggregates of SWNTs were formed in the T30-SWNT hybrids, even at 54°C, although the <jats:italic>φ</jats:italic>30-SWNT hybrids were stable up to 84°C. Second, we added secondary DNA molecules during the annealing procedure. We reacted adenine 30-mers (A30) with the T30-SWNT hybrids and characterized the samples by combining agarose gel electrophoresis with/without ethidium bromide and atomic force microscopy (AFM) as well as near-infrared photoluminescence (PL) spectroscopy. Cross-links appeared to form among the SWNTs because of nonspecific hybridization of T30 and A30. PL measurements revealed clear shifts in the PL emission wavelength of SWNTs. However, when complementary <jats:italic>φ</jats:italic>30 DNA (c<jats:italic>φ</jats:italic>30) was reacted with <jats:italic>φ</jats:italic>30-SWNT hybrids, there was no significant difference in the PL spectra after the reaction, although electrophoresis suggested the hybridization of the c<jats:italic>φ</jats:italic>30 and <jats:italic>φ</jats:italic>30 DNA molecules. Our results suggest that the hybridization manner of DNA molecules with unnatural sequences greatly differs from that of natural DNA molecules.
In application/xml+jats format

Archived Files and Locations

application/pdf  5.8 MB
file_i6kui36y3be3hfsnwzruqfom5a
downloads.hindawi.com (publisher)
web.archive.org (webarchive)
Read Archived PDF
Preserved and Accessible
Type  article-journal
Stage   published
Date   2019-04-11
Language   en ?
Journal Metadata
Open Access Publication
In DOAJ
In ISSN ROAD
In Keepers Registry
ISSN-L:  1687-4110
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: de0965cf-336e-45e6-bf46-10730ffbba91
API URL: JSON