Changes of Nicotinamide Phosphoribosyltransferase Expressions in Thyroid Glands of Patients with Different Thyroid Pathologies release_gges6kzvsvfvxkbusr6w6o5ymq

by Nadia Sawicka-Gutaj, Mirosław Andrusiewicz, Agata Czarnywojtek, Joanna Waligórska-Stachura, Maciej Biczysko, Jerzy Skrobisz, Jerzy Sowiński, Marek Ruchała

Published in BioMed Research International by Hindawi Limited.

2018   Volume 2018, p1-6

Abstract

<jats:italic>Purpose</jats:italic>. Our aim was to analyze NAMPT expression in thyroid tissue derived from patients with Graves' disease with (GD) and without (GO) orbitopathy, patients with toxic nodular goiters (TNG) and thyroid cancers (TC), and healthy controls.<jats:italic> Methods</jats:italic>. 153 thyroid tissue samples of consecutive patients who underwent thyroidectomy were collected. Previous therapy with steroids was an exclusion criterion. We collected clinicopathological data of all subjects and we assessed NAMPT expression using qPCR.<jats:italic> Results</jats:italic>. We found the highest NAMPT expression in the thyroids of patients with GO (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:mi>n</mml:mi></mml:mrow></mml:math> = 20) and cancers (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M2"><mml:mrow><mml:mi>n</mml:mi></mml:mrow></mml:math> = 40). Also, there was statistically significant NAMPT overexpression in patients with TNG (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M3"><mml:mrow><mml:mi>n</mml:mi></mml:mrow></mml:math> = 30). Relatively low NAMPT expression was found in GD patients (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M4"><mml:mrow><mml:mi>n</mml:mi></mml:mrow></mml:math> = 21) and in the control group (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M5"><mml:mrow><mml:mi>n</mml:mi></mml:mrow></mml:math> = 39). In one-way ANCOVA, we confirmed that NAMPT expression differs between subgroups and that it is not influenced by age, BMI, or sex of patients.<jats:italic> Conclusions</jats:italic>. Reported alteration of NAMPT expression might suggest its involvement in thyroid pathologies. Observed NAMPT overexpression in patients with GO and its relatively low levels in thyroids of patients with GD without eye changes do not confirm causal relationship between NAMPT level and orbitopathy, but this needs further investigation.
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Type  article-journal
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Year   2018
Language   en ?
DOI  10.1155/2018/1316390
PubMed  29546048
PMC  PMC5818968
Wikidata  Q52726197
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