Mast cell differentiation of leukemic blasts in diverse myeloid neoplasms: A potential pre‐myelomastocytic leukemia condition release_fygjjpwb2bbihazouxuo7vpwpm

by Devasis Panda, Gaurav Chatterjee, Twinkle Khanka, Sitaram Ghogale, Yajamanam Badrinath, Nilesh Deshpande, Rohan Sardana, Anumeha Chaturvedi, Sweta Rajpal, Dhanalaxmi Shetty, Nikhil V. Patkar, Sumeet Gujral (+2 others)

Published in Cytometry. Part B, Clinical cytometry by Wiley.

2020  

Abstract

Myeloid neoplasm with blasts showing mast cell (MC)-differentiation and MC-component less than 10% of all nucleated cells but not fulfilling the criteria for systemic mastocytosis with associated hematological neoplasm (SM-AHN) or myelomastocytic leukemia (MML) has not been described in the literature. Herein, we report a study of diverse myeloid malignancies with blasts showing MC-differentiation but not meeting the criteria for SM-AHN or MML. We also evaluated the utility of flow-cytometric immunophenotyping (FCI) in the characterization of immature-MCs (iMCs). We identified nine patients of myeloid neoplasms and studied their morphological, FCI, immunohistochemistry, cytogenetic and molecular characteristics. We also compared the immunophenotypic features of MCs from patient samples with control samples. The study included patients with newly-diagnosed acute myeloid leukemia (n = 4), chronic myelomonocytic leukemia (n = 1), and chronic myeloid leukemia on follow-up (n = 4) showing MC differentiation in leukemic-blasts. These patients had mildly increased MCs (range, 0.5%-3%) in bone-marrow morphology, including immature-forms and did not meet the criteria for either SM-AHN or MML. On FCI, iMCs were positive for bright-CD117, heterogeneous-CD34, dim-to-negative-HLADR, and moderate-CD203c expression. Expression-levels of CD123 and CD38 were higher (p < 0.001) but CD33 and CD45 were lower in iMCs compared to mature-MC from control samples (p = 0.019 and p = 0.0037). We reported a rare finding of MC differentiation of leukemic blasts in diverse myeloid neoplasms and proposed it as a potential pre-myelomastocytic leukemia condition. We described the distinct immunophenotypic signature of immature-MCs using commonly used markers and highlighted the utility of FCI for the diagnosis of this entity.
In text/plain format

Archived Content

There are no accessible files associated with this release. You could check other releases for this work for an accessible version.

"Dark" Preservation Only
Save Paper Now!

Know of a fulltext copy of on the public web? Submit a URL and we will archive it

Type  article-journal
Stage   published
Date   2020-08-01
Language   en ?
Container Metadata
Not in DOAJ
In Keepers Registry
ISSN-L:  1552-4949
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: 06239077-2aad-43ea-9647-b6c993c7132f
API URL: JSON