Alpha-synuclein oligomers alter the spontaneous firing discharge of cultured midbrain neurons release_f72pgswi65gnxb3po32diyo4pe

by Giulia Tomagra, Claudio Franchino, Federico Cesano, Giovanni Chiarion, Antonio de lure, Emilio Carbone, Paolo Calabresi, Luca Mesin, Barbara Picconi, Andrea Marcantoni, Valentina Carabelli

Entity Metadata (schema)

abstracts[] {'sha1': 'b9d0ca547618ddac808d0d3dfd6dadd9d36eb6c0', 'content': 'The aim of this work was to monitor the effects of extracellular α-synuclein on the firing activity of midbrain neurons dissociated from substantia nigra TH-GFP mice embryos and cultured on microelectrode arrays (MEA). We monitored the spontaneous firing discharge of the network for 21 days after plating and the role of glutamatergic and GABAergic inputs in regulating burst generation and network synchronism. Addition of GABA<jats:sub><jats:italic>A</jats:italic></jats:sub>, AMPA and NMDA antagonists did not suppress the spontaneous activity but allowed to identify three types of neurons that exhibited different modalities of firing and response to applied L-DOPA: high-rate (HR) neurons, low-rate pacemaking (LR-p), and low-rate non-pacemaking (LR-np) neurons. Most HR neurons were insensitive to L-DOPA, while the majority of LR-p neurons responded with a decrease of the firing discharge; less defined was the response of LR-np neurons. The effect of exogenous α-synuclein (α-syn) on the firing discharge of midbrain neurons was then studied by varying the exposure time (0–48 h) and the α-syn concentration (0.3–70 μM), while the formation of α-syn oligomers was monitored by means of AFM. Independently of the applied concentration, acute exposure to α-syn monomers did not exert any effect on the spontaneous firing rate of HR, LR-p, and LR-np neurons. On the contrary, after 48 h exposure, the firing activity was drastically altered at late developmental stages (14 days <jats:italic>in vitro</jats:italic>, DIV, neurons): α-syn oligomers progressively reduced the spontaneous firing discharge (IC<jats:sub>50</jats:sub> = 1.03 μM), impaired burst generation and network synchronism, proportionally to the increased oligomer/monomer ratio. Different effects were found on early-stage developed neurons (9 DIV), whose firing discharge remained unaltered, regardless of the applied α-syn concentration and the exposure time. Our findings unravel, for the first time, the variable effects of exogenous α-syn at different stages of midbrain network development and provide new evidence for the early detection of neuronal function impairment associated to aggregated forms of α-syn.', 'mimetype': 'application/xml+jats', 'lang': None}
container {'state': 'active', 'ident': 'n5oku2uutza7dou2nqqzxhetqy', 'revision': '26e1b2c7-aa10-4e1e-a68a-78e1d60b684b', 'redirect': None, 'extra': {'abbrev': 'Front Cell Neurosci', 'country': 'ch', 'default_license': 'CC-BY', 'doaj': {'archive': ['CLOCKSS'], 'as_of': '2022-07-06', 'default_license': 'CC-BY', 'seal': True}, 'kbart': {'clockss': {'year_spans': [[2009, 2022]]}}, 'languages': ['en'], 'publisher_type': 'oa', 'road': {'as_of': '2018-01-24'}, 'sherpa_romeo': {'color': 'green'}, 'urls': ['', '', '']}, 'edit_extra': None, 'name': 'Frontiers in Cellular Neuroscience', 'container_type': None, 'publication_status': None, 'publisher': 'Frontiers Media SA', 'issnl': '1662-5102', 'issne': '1662-5102', 'issnp': '1662-5102', 'wikidata_qid': 'Q2131509'}
container_id n5oku2uutza7dou2nqqzxhetqy
contribs[] {'index': 0, 'creator_id': None, 'creator': None, 'raw_name': 'Giulia Tomagra', 'given_name': 'Giulia', 'surname': 'Tomagra', 'role': 'author', 'raw_affiliation': None, 'extra': {'seq': 'first'}}
{'index': 1, 'creator_id': None, 'creator': None, 'raw_name': 'Claudio Franchino', 'given_name': 'Claudio', 'surname': 'Franchino', 'role': 'author', 'raw_affiliation': None, 'extra': None}
{'index': 2, 'creator_id': None, 'creator': None, 'raw_name': 'Federico Cesano', 'given_name': 'Federico', 'surname': 'Cesano', 'role': 'author', 'raw_affiliation': None, 'extra': None}
{'index': 3, 'creator_id': None, 'creator': None, 'raw_name': 'Giovanni Chiarion', 'given_name': 'Giovanni', 'surname': 'Chiarion', 'role': 'author', 'raw_affiliation': None, 'extra': None}
{'index': 4, 'creator_id': None, 'creator': None, 'raw_name': 'Antonio de lure', 'given_name': 'Antonio', 'surname': 'de lure', 'role': 'author', 'raw_affiliation': None, 'extra': None}
{'index': 5, 'creator_id': None, 'creator': None, 'raw_name': 'Emilio Carbone', 'given_name': 'Emilio', 'surname': 'Carbone', 'role': 'author', 'raw_affiliation': None, 'extra': None}
{'index': 6, 'creator_id': None, 'creator': None, 'raw_name': 'Paolo Calabresi', 'given_name': 'Paolo', 'surname': 'Calabresi', 'role': 'author', 'raw_affiliation': None, 'extra': None}
{'index': 7, 'creator_id': None, 'creator': None, 'raw_name': 'Luca Mesin', 'given_name': 'Luca', 'surname': 'Mesin', 'role': 'author', 'raw_affiliation': None, 'extra': None}
{'index': 8, 'creator_id': None, 'creator': None, 'raw_name': 'Barbara Picconi', 'given_name': 'Barbara', 'surname': 'Picconi', 'role': 'author', 'raw_affiliation': None, 'extra': None}
{'index': 9, 'creator_id': None, 'creator': None, 'raw_name': 'Andrea Marcantoni', 'given_name': 'Andrea', 'surname': 'Marcantoni', 'role': 'author', 'raw_affiliation': None, 'extra': None}
{'index': 10, 'creator_id': None, 'creator': None, 'raw_name': 'Valentina Carabelli', 'given_name': 'Valentina', 'surname': 'Carabelli', 'role': 'author', 'raw_affiliation': None, 'extra': None}
ext_ids {'doi': '10.3389/fncel.2023.1078550', 'wikidata_qid': None, 'isbn13': None, 'pmid': '36744002', 'pmcid': 'PMC9896582', 'core': None, 'arxiv': None, 'jstor': None, 'ark': None, 'mag': None, 'doaj': None, 'dblp': None, 'oai': None, 'hdl': None}
files[] {'state': 'active', 'ident': 'jsewpmqcpbaajhtbjfhnfpua3m', 'revision': 'eb852a28-9284-46d9-977b-fcb15dc65753', 'redirect': None, 'extra': None, 'edit_extra': None, 'size': 9494799, 'md5': '87571cec8af0b62e21cd71d9687467eb', 'sha1': '6a36cbdf46f678b5d1f6c9d429cc0d6b93f57d82', 'sha256': '8a8b7e7712799f3c56de4ca882d9bfb44c530ad455249982da6e29c4e153575a', 'urls': [{'url': '', 'rel': 'publisher'}, {'url': '', 'rel': 'webarchive'}], 'mimetype': 'application/pdf', 'content_scope': None, 'release_ids': ['f72pgswi65gnxb3po32diyo4pe'], 'releases': None}
filesets []
language en
license_slug CC-BY
pages 1078550
publisher Frontiers Media SA
refs[] {'index': 0, 'target_release_id': None, 'extra': {'authors': ['Abeles'], 'doi': '10.1016/0165-0270(82)90002-4'}, 'key': 'B1', 'year': 1982, 'container_name': 'J. Neurosci. Methods', 'title': "Quantification, smoothing, and confidence limits for single-units' histograms.", 'locator': '317'}
{'index': 1, 'target_release_id': None, 'extra': {'authors': ['Allio'], 'doi': '10.1016/j.jep.2015.06.016', 'volume': '172'}, 'key': 'B2', 'year': 2015, 'container_name': 'J. Ethnopharmacol.', 'title': 'Bud extracts from Tilia tomentosa Moench inhibit hippocampal neuronal firing through GABAA and benzodiazepine receptors activation.', 'locator': '288'}
{'index': 2, 'target_release_id': None, 'extra': {'authors': ['Aversa'], 'doi': '10.1111/bph.14422', 'volume': '175'}, 'key': 'B3', 'year': 2018, 'container_name': 'Br. J. Pharmacol.', 'title': 'Reversal of dopamine-mediated firing inhibition through activation of the dopamine transporter in substantia nigra pars compacta neurons.', 'locator': '3534'}
{'index': 3, 'target_release_id': None, 'extra': {'authors': ['Bar-Gad'], 'doi': '10.1016/s0165-0270(00)00335-6', 'volume': '104'}, 'key': 'B4', 'year': 2001, 'container_name': 'J. Neurosci. Methods', 'title': 'The neuronal refractory period causes a short-term peak in the autocorrelation function.', 'locator': '155'}
{'index': 4, 'target_release_id': None, 'extra': {'authors': ['Berretta'], 'doi': '10.1113/jphysiol.2010.189415'}, 'key': 'B5', 'year': 2010, 'container_name': 'J. Physiol.', 'title': 'Firing properties and functional connectivity of substantia nigra pars compacta neurones recorded with a multi-electrode array in vitro.', 'locator': '1719'}
{'index': 5, 'target_release_id': None, 'extra': {'authors': ['Berretta'], 'doi': '10.1016/j.neuro.2005.01.014', 'volume': '26'}, 'key': 'B6', 'year': 2005, 'container_name': 'Neurotoxicology', 'title': 'Acute effects of 6-hydroxydopamine on dopaminergic neurons of the rat substantia nigra pars compacta in vitro.', 'locator': '869'}
{'index': 6, 'target_release_id': None, 'extra': {'authors': ['Bhak'], 'doi': '10.1038/s41598-018-32655-0'}, 'key': 'B7', 'year': 2018, 'container_name': 'Sci. Rep.', 'title': 'Morphological evaluation of meta-stable oligomers of α-synuclein with small-angle neutron scattering.', 'locator': None}
{'index': 7, 'target_release_id': None, 'extra': {'authors': ['Blythe'], 'doi': '10.1523/jneurosci.2961-09.2009', 'volume': '29'}, 'key': 'B8', 'year': 2009, 'container_name': 'J. Neurosci.', 'title': 'Cellular mechanisms underlying burst firing in substantia nigra dopamine neurons.', 'locator': '15531'}
{'index': 8, 'target_release_id': None, 'extra': {'authors': ['Borodich'], 'doi': '10.3389/fmech.2020.00064'}, 'key': 'B9', 'year': 2020, 'container_name': 'Front. Mech. Eng.', 'title': 'Probabilistic, fractal, and related techniques for analysis of engineering surfaces.', 'locator': None}
{'index': 9, 'target_release_id': None, 'extra': {'authors': ['Braak'], 'doi': '10.1016/s0197-4580(02)00065-9', 'volume': '24'}, 'key': 'B10', 'year': 2003, 'container_name': 'Neurobiol. Aging', 'title': "Staging of brain pathology related to sporadic Parkinson's disease.", 'locator': '197'}
{'index': 10, 'target_release_id': None, 'extra': {'authors': ['Bridi'], 'doi': '10.3389/fnins.2018.00080', 'volume': '12'}, 'key': 'B11', 'year': 2018, 'container_name': 'Front. Neurosci.', 'title': "Mechanisms of alpha-synuclein induced synaptopathy in Parkinson's Disease.", 'locator': None}
{'index': 11, 'target_release_id': None, 'extra': {'authors': ['Bullmore'], 'doi': '10.1038/nrn2575', 'volume': '10'}, 'key': 'B12', 'year': 2009, 'container_name': 'Nat. Rev. Neurosci.', 'title': 'Complex brain networks: Graph theoretical analysis of structural and functional systems.', 'locator': '186'}
{'index': 12, 'target_release_id': None, 'extra': {'authors': ['Carbone'], 'doi': '10.3389/fncel.2017.00187', 'volume': '11'}, 'key': 'B13', 'year': 2017, 'container_name': 'Front. Cell. Neurosci.', 'title': 'The Hyperpolarization-activated current determines synaptic excitability, calcium activity and specific viability of substantia nigra dopaminergic neurons.', 'locator': None}
{'index': 13, 'target_release_id': None, 'extra': {'authors': ['Chen'], 'doi': '10.1016/j.pnsc.2008.05.027', 'volume': '19'}, 'key': 'B14', 'year': 2009, 'container_name': 'Prog. Nat. Sci.', 'title': 'Detection of bursts in neuronal spike trains by the mean inter-spike interval method.', 'locator': '229'}
{'index': 14, 'target_release_id': None, 'extra': {'authors': ['Chergui'], 'doi': '10.1111/j.1460-9568.1993.tb00479.x'}, 'key': 'B15', 'year': 1993, 'container_name': 'Eur. J. Neurosci.', 'title': 'Tonic activation of NMDA receptors causes spontaneous burst discharge of rat midbrain dopamine neurons in vivo.', 'locator': '137'}
{'index': 15, 'target_release_id': None, 'extra': {'authors': ['Chiarion'], 'volume': '10'}, 'key': 'B16', 'year': None, 'container_name': 'Electronics', 'title': 'Functional connectivity of EEG in encephalitis during slow biphasic complexes.', 'locator': None}
{'index': 16, 'target_release_id': None, 'extra': {'authors': ['Chiarion'], 'volume': '10'}, 'key': 'B17', 'year': None, 'container_name': 'Electronics', 'title': 'Resolution of spike overlapping by biogeography-based optimization.', 'locator': None}
{'index': 17, 'target_release_id': None, 'extra': {'authors': ['Cohen'], 'doi': '10.1016/j.brainres.2008.06.022', 'volume': '1235'}, 'key': 'B18', 'year': 2008, 'container_name': 'Brain Res.', 'title': 'Determinants of spontaneous activity in networks of cultured hippocampus.', 'locator': '21'}
{'index': 18, 'target_release_id': None, 'extra': {'authors': ['Cotterill'], 'doi': '10.1007/978-3-030-11135-9_8', 'volume': '22'}, 'key': 'B19', 'year': 2019, 'container_name': 'Adv. Neurobiol.', 'title': 'Burst detection methods.', 'locator': '185'}
{'index': 19, 'target_release_id': None, 'extra': {'authors': ['Dagra'], 'doi': '10.1038/s41531-021-00210-w'}, 'key': 'B20', 'year': 2021, 'container_name': 'NPJ Parkinsons Dis.', 'title': 'α-Synuclein-induced dysregulation of neuronal activity contributes to murine dopamine neuron vulnerability.', 'locator': None}
{'index': 20, 'target_release_id': None, 'extra': {'authors': ['Danzer'], 'doi': '10.1186/1750-1326-7-42'}, 'key': 'B21', 'year': 2012, 'container_name': 'Mol. Neurodegen.', 'title': 'Exosomal cell-to-cell transmission of alpha synuclein oligomers.', 'locator': None}
{'index': 21, 'target_release_id': None, 'extra': {'authors': ['Dauer'], 'doi': '10.1016/s0896-6273(03)00568-3', 'volume': '39'}, 'key': 'B22', 'year': 2003, 'container_name': 'Neuron', 'title': "Parkinson's Disease: Mechanisms and models.", 'locator': '889'}
{'index': 22, 'target_release_id': None, 'extra': {'authors': ['Del Pino'], 'doi': '10.1093/cercor/bhaa137', 'volume': '30'}, 'key': 'B23', 'year': 2020, 'container_name': 'Cereb. Cortex', 'title': 'COUP-TFI/Nr2f1 orchestrates intrinsic neuronal activity during development of the somatosensory cortex.', 'locator': '5667'}
{'index': 23, 'target_release_id': None, 'extra': {'authors': ['Dobi'], 'doi': '10.1523/jneurosci.3884-09.2010', 'volume': '30'}, 'key': 'B24', 'year': 2010, 'container_name': 'J. Neurosci.', 'title': 'Glutamatergic and nonglutamatergic neurons of the ventral tegmental area establish local synaptic contacts with dopaminergic and nondopaminergic neurons.', 'locator': '218'}
{'index': 24, 'target_release_id': None, 'extra': {'authors': ['Durante'], 'doi': '10.1093/brain/awz065', 'volume': '142'}, 'key': 'B25', 'year': 2019, 'container_name': 'Brain', 'title': 'Alpha-synuclein targets GluN2A NMDA receptor subunit causing striatal synaptic dysfunction and visuospatial memory alteration.', 'locator': '1365'}
{'index': 25, 'target_release_id': None, 'extra': {'authors': ['El-Agnaf'], 'doi': '10.1096/fj.03-1449com', 'volume': '20'}, 'key': 'B26', 'year': 2006, 'container_name': 'FASEB J.', 'title': "Detection of oligomeric forms of alpha-synuclein protein in human plasma as a potential biomarker for Parkinson's disease.", 'locator': '419'}
{'index': 26, 'target_release_id': None, 'extra': {'authors': ['Emadi'], 'doi': '10.1074/jbc.m806559200', 'volume': '284'}, 'key': 'B27', 'year': 2009, 'container_name': 'J. Biol. Chem.', 'title': 'Detecting morphologically distinct oligomeric forms of alpha-synuclein.', 'locator': '11048'}
{'index': 27, 'target_release_id': None, 'extra': {'authors': ['Emanuele'], 'doi': '10.1016/j.ebiom.2016.03.038'}, 'key': 'B28', 'year': 2016, 'container_name': 'EBioMedicine', 'title': 'Exogenous alpha-synuclein alters pre- and post-synaptic activity by fragmenting lipid rafts.', 'locator': '191'}
{'index': 28, 'target_release_id': None, 'extra': {'authors': ['Emmanouilidou'], 'doi': '10.1111/bpa.12373', 'volume': '26'}, 'key': 'B29', 'year': 2016, 'container_name': 'Brain Pathol.', 'title': 'Exocytosis and spreading of normal and aberrant alpha-synuclein.', 'locator': '398'}
{'index': 29, 'target_release_id': None, 'extra': {'authors': ['Eusebi'], 'doi': '10.1002/mds.27110', 'volume': '32'}, 'key': 'B30', 'year': 2017, 'container_name': 'Mov. Disord', 'title': "Diagnostic utility of cerebrospinal fluid α-synuclein in Parkinson's disease: A systematic review and meta-analysis.", 'locator': '1389'}
{'index': 30, 'target_release_id': None, 'extra': {'authors': ['Fath'], 'doi': '10.1038/nprot.2008.199'}, 'key': 'B31', 'year': 2009, 'container_name': 'Nat. Protocols', 'title': 'Primary support cultures of hippocampal and substantia nigra neurons.', 'locator': '78'}
{'index': 31, 'target_release_id': None, 'extra': {'authors': ['Froula'], 'doi': '10.1186/s40478-018-0537-x'}, 'key': 'B32', 'year': 2018, 'container_name': 'Acta Neuropathol. Commun.', 'title': 'α-Synuclein fibril-induced paradoxical structural and functional defects in hippocampal neurons.', 'locator': None}
{'index': 32, 'target_release_id': None, 'extra': {'authors': ['Garcia-Reitboeck'], 'doi': '10.1016/j.expneurol.2013.07.015', 'volume': '248'}, 'key': 'B33', 'year': 2013, 'container_name': 'Exp. Neurol.', 'title': 'Endogenous alpha-synuclein influences the number of dopaminergic neurons in mouse substantia nigra.', 'locator': '541'}
{'index': 33, 'target_release_id': None, 'extra': {'authors': ['Gavello'], 'doi': '10.1093/cercor/bhw377', 'volume': '28'}, 'key': 'B34', 'year': 2018, 'container_name': 'Cereb. Cortex', 'title': 'Early alterations of hippocampal neuronal firing induced by Abeta42.', 'locator': '433'}
{'index': 34, 'target_release_id': None, 'extra': {'authors': ['Gavello'], 'doi': '10.1371/journal.pone.0041530'}, 'key': 'B35', 'year': 2012, 'container_name': 'PLoS One', 'title': 'Leptin counteracts the hypoxia-induced inhibition of spontaneously firing hippocampal neurons: A microelectrode array study.', 'locator': None}
{'index': 35, 'target_release_id': None, 'extra': {'authors': ['Gaven'], 'doi': '10.3791/51751'}, 'key': 'B36', 'year': 2014, 'container_name': 'JoVE', 'title': 'Primary culture of mouse dopaminergic neurons.', 'locator': None}
{'index': 36, 'target_release_id': None, 'extra': {'authors': ['Georges'], 'doi': '10.1523/jneurosci.22-12-05173.2002', 'volume': '22'}, 'key': 'B37', 'year': 2002, 'container_name': 'J. Neurosci.', 'title': 'Activation of ventral tegmental area cells by the bed nucleus of the stria terminalis: A novel excitatory amino acid input to midbrain dopamine neurons.', 'locator': '5173'}
{'index': 37, 'target_release_id': None, 'extra': {'authors': ['Ghiglieri'], 'doi': '10.3389/fneur.2018.00295'}, 'key': 'B38', 'year': 2018, 'container_name': 'Front. Neurol.', 'title': 'Alpha-synuclein: From early synaptic dysfunction to neurodegeneration.', 'locator': None}
{'index': 38, 'target_release_id': None, 'extra': {'authors': ['Gold'], 'doi': '10.1152/jn.00979.2005', 'volume': '95'}, 'key': 'B39', 'year': 2006, 'container_name': 'J. Neurophysiol.', 'title': 'On the origin of the extracellular action potential waveform: A modeling study.', 'locator': '3113'}
{'index': 39, 'target_release_id': None, 'extra': {'authors': ['Guatteo'], 'doi': '10.1016/j.expneurol.2013.02.009', 'volume': '247'}, 'key': 'B40', 'year': 2013, 'container_name': 'Exp. Neurol.', 'title': 'Dual effects of L-DOPA on nigral dopaminergic neurons.', 'locator': '582'}
{'index': 40, 'target_release_id': None, 'extra': {'authors': ['Hassink'], 'doi': '10.1371/journal.pone.0193763', 'volume': '13'}, 'key': 'B41', 'year': 2018, 'container_name': 'PLoS One', 'title': 'Exogenous α-synuclein hinders synaptic communication in cultured cortical primary rat neurons.', 'locator': None}
{'index': 41, 'target_release_id': None, 'extra': {'authors': ['Hill'], 'doi': '10.1523/eneuro.0330-20.2020'}, 'key': 'B42', 'year': 2021, 'container_name': 'eneuro', 'title': 'α-Synuclein aggregates increase the conductance of substantia nigra dopamine neurons, an effect partly reversed by the KATP channel inhibitor glibenclamide.', 'locator': None}
{'index': 42, 'target_release_id': None, 'extra': {'authors': ['Hofmann'], 'doi': '10.1016/j.jphysparis.2005.12.005', 'volume': '99'}, 'key': 'B43', 'year': 2006, 'container_name': 'J. Physiol.', 'title': 'Long term recordings with microelectrode arrays: Studies of transcription-dependent neuronal plasticity and axonal regeneration.', 'locator': '125'}
{'index': 43, 'target_release_id': None, 'extra': {'authors': ['Houlden'], 'doi': '10.1007/s00401-012-1013-5', 'volume': '124'}, 'key': 'B44', 'year': 2012, 'container_name': 'Acta Neuropathol.', 'title': "The genetics and neuropathology of Parkinson's disease.", 'locator': '325'}
{'index': 44, 'target_release_id': None, 'extra': {'authors': ['Iversen'], 'doi': '10.1016/j.tins.2007.03.002', 'volume': '30'}, 'key': 'B45', 'year': 2007, 'container_name': 'Trends Neurosci.', 'title': 'Dopamine: 50 years in perspective.', 'locator': '188'}
{'index': 45, 'target_release_id': None, 'extra': {'authors': ['Johnson'], 'doi': '10.1126/science.1329209', 'volume': '258'}, 'key': 'B46', 'year': 1992, 'container_name': 'Science', 'title': 'Burst firing in dopamine neurons induced by N-methyl-D-aspartate: Role of electrogenic sodium pump.', 'locator': '665'}
{'index': 46, 'target_release_id': None, 'extra': {'authors': ['Jurjuţ'], 'doi': '10.3389/fnsys.2019.00021', 'volume': '13'}, 'key': 'B47', 'year': 2019, 'container_name': 'Front. Syst. Neurosci.', 'title': 'Hold your methods! how multineuronal firing ensembles can be studied using classical spike-train analysis techniques.', 'locator': None}
{'index': 47, 'target_release_id': None, 'extra': {'authors': ['Kitai'], 'doi': '10.1016/s0959-4388(99)00040-9'}, 'key': 'B48', 'year': 1999, 'container_name': 'Curr. Opin. Neurobiol.', 'title': 'Afferent modulation of dopamine neuron firing patterns.', 'locator': '690'}
{'index': 48, 'target_release_id': None, 'extra': {'authors': ['Koob'], 'doi': '10.1016/s0896-6273(00)80557-7', 'volume': '21'}, 'key': 'B49', 'year': 1998, 'container_name': 'Neuron', 'title': 'Neuroscience of addiction.', 'locator': '467'}
{'index': 49, 'target_release_id': None, 'extra': {'authors': ['Lacey'], 'doi': '10.1113/jphysiol.1987.sp016787', 'volume': '392'}, 'key': 'B50', 'year': 1987, 'container_name': 'J. Physiol.', 'title': 'Dopamine acts on D2 receptors to increase potassium conductance in neurones of the rat substantia nigra zona compacta.', 'locator': '397'}
{'index': 50, 'target_release_id': None, 'extra': {'authors': ['Lacey'], 'doi': '10.1523/jneurosci.09-04-01233.1989'}, 'key': 'B51', 'year': 1989, 'container_name': 'J. Neurosci.', 'title': 'Two cell types in rat substantia nigra zona compacta distinguished by membrane properties and the actions of dopamine and opioids.', 'locator': '1233'}
{'index': 51, 'target_release_id': None, 'extra': {'authors': ['Lewis'], 'doi': '10.1016/s0896-6273(00)00111-2', 'volume': '28'}, 'key': 'B52', 'year': 2000, 'container_name': 'Neuron', 'title': 'Catching up on schizophrenia: Natural history and neurobiology.', 'locator': '325'}
{'index': 52, 'target_release_id': None, 'extra': {'authors': ['Lin'], 'doi': '10.1186/s40478-021-01203-9'}, 'key': 'B53', 'year': 2021, 'container_name': 'Acta Neuropathol. Commun.', 'title': "In Parkinson's patient-derived dopamine neurons, the triplication of α-synuclein locus induces distinctive firing pattern by impeding D2 receptor autoinhibition.", 'locator': None}
{'index': 53, 'target_release_id': None, 'extra': {'authors': ['Lozano'], 'doi': '10.1038/s41598-019-50745-5'}, 'key': 'B54', 'year': 2019, 'container_name': 'Sci. Rep.', 'title': 'Sizing single nanoscale objects from polarization forces.', 'locator': None}
{'index': 54, 'target_release_id': None, 'extra': {'authors': ['Maex'], 'doi': '10.1111/j.1469-7793.2000.t01-1-00175.x', 'volume': '523'}, 'key': 'B55', 'year': 2000, 'container_name': 'J. Physiol.', 'title': 'Weak common parallel fibre synapses explain the loose synchrony observed between rat cerebellar Golgi cells.', 'locator': '175'}
{'index': 55, 'target_release_id': None, 'extra': {'authors': ['Mannal'], 'doi': '10.3389/fnsyn.2021.635050', 'volume': '13'}, 'key': 'B56', 'year': 2021, 'container_name': 'Front. Synaptic Neurosci.', 'title': 'Multi-electrode array analysis identifies complex dopamine responses and glucose sensing properties of substantia nigra neurons in mouse brain slices.', 'locator': None}
{'index': 56, 'target_release_id': None, 'extra': {'authors': ['Marcantoni'], 'doi': '10.1007/s00424-022-02769-6'}, 'key': 'B57', 'year': 2022, 'container_name': 'Pflugers Arch.', 'title': 'Correction to: Two firing modes and well-resolved Na(+), K(+), and Ca(2+) currents at the cell-microelectrode junction of spontaneously active rat chromaffin cell on MEAs.', 'locator': None}
{'index': 57, 'target_release_id': None, 'extra': {'authors': ['Matsushita'], 'doi': '10.1046/j.1471-4159.2002.00972.x', 'volume': '82'}, 'key': 'B58', 'year': 2002, 'container_name': 'J. Neurochem.', 'title': 'Dynamics of tyrosine hydroxylase promoter activity during midbrain dopaminergic neuron development.', 'locator': '295'}
{'index': 58, 'target_release_id': None, 'extra': {'authors': ['McNaught'], 'doi': '10.1016/j.neurobiolaging.2005.08.012', 'volume': '27'}, 'key': 'B59', 'year': 2006, 'container_name': 'Neurobiol. Aging', 'title': "Protein aggregation in the pathogenesis of familial and sporadic Parkinson's disease.", 'locator': '530'}
{'index': 59, 'target_release_id': None, 'extra': {'authors': ['Mercuri'], 'doi': '10.1111/j.1476-5381.1990.tb15792.x', 'volume': '100'}, 'key': 'B60', 'year': 1990, 'container_name': 'Br. J. Pharmacol.', 'title': 'Responses of rat substantia nigra compacta neurones to L-DOPA.', 'locator': '257'}
{'index': 60, 'target_release_id': None, 'extra': {'authors': ['Moore'], 'doi': '10.1016/s0006-3495(70)86341-x', 'volume': '10'}, 'key': 'B61', 'year': 1970, 'container_name': 'Biophys. J.', 'title': 'Statistical signs of synaptic interaction in neurons.', 'locator': '876'}
{'index': 61, 'target_release_id': None, 'extra': {'authors': ['Neèas'], 'doi': '10.2478/s11534-011-0096-2', 'volume': '10'}, 'key': 'B62', 'year': 2012, 'container_name': 'Open Phys.', 'title': 'Gwyddion: An open-source software for SPM data analysis.', 'locator': '181'}
{'index': 62, 'target_release_id': None, 'extra': {'authors': ['Nieoullon'], 'doi': '10.1016/s0301-0082(02)00011-4', 'volume': '67'}, 'key': 'B63', 'year': 2002, 'container_name': 'Prog. Neurobiol.', 'title': 'Dopamine and the regulation of cognition and attention.', 'locator': '53'}
{'index': 63, 'target_release_id': None, 'extra': {'authors': ['Opalka'], 'doi': '10.1016/j.celrep.2019.12.038', 'volume': '30'}, 'key': 'B64', 'year': 2020, 'container_name': 'Cell Rep.', 'title': 'Hippocampal ripple coordinates retrosplenial inhibitory neurons during slow-wave sleep.', 'locator': '432.e'}
{'index': 64, 'target_release_id': None, 'extra': {'authors': ['Peyrache'], 'doi': '10.1073/pnas.1109895109', 'volume': '109'}, 'key': 'B65', 'year': 2012, 'container_name': 'Proc. Natl. Acad. Sci. U.S.A.', 'title': 'Spatiotemporal dynamics of neocortical excitation and inhibition during human sleep.', 'locator': '1731'}
{'index': 65, 'target_release_id': None, 'extra': {'authors': ['Poulin'], 'doi': '10.1038/s41593-018-0203-4', 'volume': '21'}, 'key': 'B66', 'year': 2018, 'container_name': 'Nat. Neurosci.', 'title': 'Mapping projections of molecularly defined dopamine neuron subtypes using intersectional genetic approaches.', 'locator': '1260'}
{'index': 66, 'target_release_id': None, 'extra': {'authors': ['Prisco'], 'doi': '10.1016/s0028-3908(01)00192-7', 'volume': '42'}, 'key': 'B67', 'year': 2002, 'container_name': 'Neuropharmacology', 'title': 'Group I metabotropic glutamate receptors activate burst firing in rat midbrain dopaminergic neurons.', 'locator': '289'}
{'index': 67, 'target_release_id': None, 'extra': {'authors': ['Qu'], 'doi': '10.1016/j.neulet.2014.05.038', 'volume': '575'}, 'key': 'B68', 'year': 2014, 'container_name': 'Neurosci. Lett.', 'title': '6-OHDA induced calcium influx through N-type calcium channel alters membrane properties via PKA pathway in substantia nigra pars compacta dopaminergic neurons.', 'locator': None}
{'index': 68, 'target_release_id': None, 'extra': {'authors': ['Rey'], 'doi': '10.1016/j.brainresbull.2015.04.007', 'volume': '119'}, 'key': 'B69', 'year': 2015, 'container_name': 'Brain Res. Bullet.', 'title': 'Past, present and future of spike sorting techniques.', 'locator': '106'}
{'index': 69, 'target_release_id': None, 'extra': {'authors': ['Rohrbacher'], 'doi': '10.1016/s0306-4522(00)00046-4', 'volume': '97'}, 'key': 'B70', 'year': 2000, 'container_name': 'Neuroscience', 'title': 'Electrophysiological characteristics of substantia nigra neurons in organotypic cultures: Spontaneous and evoked activities.', 'locator': '703'}
{'index': 70, 'target_release_id': None, 'extra': {'authors': ['Ronzitti'], 'doi': '10.1523/jneurosci.0608-14.2014', 'volume': '34'}, 'key': 'B71', 'year': 2014, 'container_name': 'J. Neurosci.', 'title': 'Exogenous alpha-synuclein decreases raft partitioning of Cav2.2 channels inducing dopamine release.', 'locator': '10603'}
{'index': 71, 'target_release_id': None, 'extra': {'authors': ['Röper'], 'doi': '10.1007/bf00373838', 'volume': '430'}, 'key': 'B72', 'year': 1995, 'container_name': 'Pflügers Arch.', 'title': 'Metabolic inhibition and low internal ATP activate K-ATP channels in rat dopaminergic substantia nigra neurones.', 'locator': '44'}
{'index': 72, 'target_release_id': None, 'extra': {'authors': ['Rubinov'], 'doi': '10.1016/j.neuroimage.2009.10.003', 'volume': '52'}, 'key': 'B73', 'year': 2010, 'container_name': 'Neuroimage', 'title': 'Complex network measures of brain connectivity: Uses and interpretations.', 'locator': '1059'}
{'index': 73, 'target_release_id': None, 'extra': {'authors': ['Sawamoto'], 'doi': '10.1073/pnas.111152398', 'volume': '98'}, 'key': 'B74', 'year': 2001, 'container_name': 'Proc. Natl. Acad. Sci. U.S.A.', 'title': 'Visualization, direct isolation, and transplantation of midbrain dopaminergic neurons.', 'locator': '6423'}
{'index': 74, 'target_release_id': None, 'extra': {'authors': ['Scarano'], 'doi': '10.1016/j.susc.2004.07.024', 'volume': '570'}, 'key': 'B75', 'year': 2004, 'container_name': 'Surface Sci.', 'title': 'Imaging polycrystalline and smoke MgO surfaces with atomic force microscopy: A case study of high resolution image on a polycrystalline oxide.', 'locator': '155'}
{'index': 75, 'target_release_id': None, 'extra': {'authors': ['Sebastianelli'], 'doi': '10.1016/j.expneurol.2008.04.005', 'volume': '212'}, 'key': 'B76', 'year': 2008, 'container_name': 'Exp. Neurol.', 'title': 'The L-amino acid carrier inhibitor 2-aminobicyclo[2.2.1]heptane-2-carboxylic acid (BCH) reduces L-dopa-elicited responses in dopaminergic neurons of the substantia nigra pars compacta.', 'locator': '230'}
{'index': 76, 'target_release_id': None, 'extra': {'authors': ['Spillantini'], 'doi': '10.1038/42166', 'volume': '388'}, 'key': 'B77', 'year': 1997, 'container_name': 'Nature', 'title': 'Alpha-synuclein in Lewy bodies.', 'locator': '839'}
{'index': 77, 'target_release_id': None, 'extra': {'authors': ['Studer'], 'doi': '10.1002/0471142301.ns0303s00'}, 'key': 'B78', 'year': 2001, 'container_name': 'Curr. Protoc. Neurosci.', 'title': 'Culture of substantia nigra neurons', 'locator': '3.3.1'}
{'index': 78, 'target_release_id': None, 'extra': {'authors': ['Subramaniam'], 'doi': '10.1523/jneurosci.5069-13.2014', 'volume': '34'}, 'key': 'B79', 'year': 2014, 'container_name': 'J. Neurosci.', 'title': 'Mutant alpha-synuclein enhances firing frequencies in dopamine substantia nigra neurons by oxidative impairment of A-type potassium channels.', 'locator': '13586'}
{'index': 79, 'target_release_id': None, 'extra': {'authors': ['Sulzer'], 'doi': '10.1016/j.baga.2016.02.001'}, 'key': 'B80', 'year': 2016, 'container_name': 'Basal Ganglia', 'title': 'Striatal dopamine neurotransmission: Regulation of release and uptake.', 'locator': '123'}
{'index': 80, 'target_release_id': None, 'extra': {'authors': ['Tepper'], 'doi': '10.1523/jneurosci.15-04-03092.1995', 'volume': '15'}, 'key': 'B81', 'year': 1995, 'container_name': 'J Neurosci', 'title': 'GABAA receptor-mediated inhibition of rat substantia nigra dopaminergic neurons by pars reticulata projection neurons.', 'locator': '3092'}
{'index': 81, 'target_release_id': None, 'extra': {'authors': ['Tomagra'], 'doi': '10.3389/fnins.2019.00288', 'volume': '13'}, 'key': 'B82', 'year': 2019, 'container_name': 'Front. Neurosci.', 'title': 'Quantal release of dopamine and action potential firing detected in midbrain neurons by multifunctional diamond-based microarrays.', 'locator': None}
{'index': 82, 'target_release_id': None, 'extra': {'authors': ['Tozzi'], 'doi': '10.1016/j.biopsych.2015.08.013', 'volume': '79'}, 'key': 'B83', 'year': 2016, 'container_name': 'Biol. Psychiatry', 'title': 'Alpha-synuclein produces early behavioral alterations via striatal cholinergic synaptic dysfunction by interacting With GluN2D N-Methyl-D-aspartate receptor subunit.', 'locator': '402'}
{'index': 83, 'target_release_id': None, 'extra': {'authors': ['Tozzi'], 'doi': '10.1093/brain/awab242', 'volume': '144'}, 'key': 'B84', 'year': 2021, 'container_name': 'Brain', 'title': 'Dopamine-dependent early synaptic and motor dysfunctions induced by α-synuclein in the nigrostriatal circuit.', 'locator': '3477'}
{'index': 84, 'target_release_id': None, 'extra': {'authors': ['van Diggelen'], 'doi': '10.1371/journal.pone.0213663', 'volume': '14'}, 'key': 'B85', 'year': 2019, 'container_name': 'PLoS One', 'title': 'Two conformationally distinct α-synuclein oligomers share common epitopes and the ability to impair long-term potentiation.', 'locator': None}
{'index': 85, 'target_release_id': None, 'extra': {'authors': ['Volpicelli-Daley'], 'doi': '10.1091/mbc.e14-02-0741', 'volume': '25'}, 'key': 'B86', 'year': 2014, 'container_name': 'Mol. Biol. Cell', 'title': 'Formation of α-synuclein Lewy neurite-like aggregates in axons impedes the transport of distinct endosomes.', 'locator': '4010'}
{'index': 86, 'target_release_id': None, 'extra': {'authors': ['Volpicelli-Daley'], 'doi': '10.1016/j.neuron.2011.08.033', 'volume': '72'}, 'key': 'B87', 'year': 2011, 'container_name': 'Neuron', 'title': 'Exogenous α-synuclein fibrils induce Lewy body pathology leading to synaptic dysfunction and neuron death.', 'locator': '57'}
{'index': 87, 'target_release_id': None, 'extra': {'authors': ['Wang'], 'doi': '10.1016/0006-8993(94)90780-3', 'volume': '666'}, 'key': 'B88', 'year': 1994, 'container_name': 'Brain Res.', 'title': 'N-methyl-D-aspartic acid biphasically regulates the biochemical and electrophysiological response of A10 dopamine neurons in the ventral tegmental area: In vivo microdialysis and in vitro electrophysiological studies.', 'locator': '255'}
{'index': 88, 'target_release_id': None, 'extra': {'authors': ['Wang'], 'doi': '10.1007/s12035-014-8728-3', 'volume': '51'}, 'key': 'B89', 'year': 2015, 'container_name': 'Mol. Neurobiol.', 'title': "Firing pattern modulation through SK channel current increase underlies neuronal survival in an organotypic slice model of Parkinson's disease.", 'locator': '424'}
{'index': 89, 'target_release_id': None, 'extra': {'authors': ['Wise'], 'doi': '10.1038/nrn1406'}, 'key': 'B90', 'year': 2004, 'container_name': 'Nat. Rev. Neurosci.', 'title': 'Dopamine, learning and motivation.', 'locator': '483'}
{'index': 90, 'target_release_id': None, 'extra': {'authors': ['Yamamoto'], 'doi': '10.1038/s41598-019-52135-3'}, 'key': 'B91', 'year': 2019, 'container_name': 'Sci. Rep.', 'title': 'α-Synuclein oligomers mediate the aberrant form of spike-induced calcium release from IP3 receptor.', 'locator': None}
{'index': 91, 'target_release_id': None, 'extra': {'authors': ['Zeldenrust'], 'doi': '10.3389/fncom.2018.00048', 'volume': '12'}, 'key': 'B92', 'year': 2018, 'container_name': 'Front. Comput. Neurosci.', 'title': 'Neural coding with bursts—current state and future perspectives.', 'locator': None}
release_date 2023-01-20
release_stage published
release_type article-journal
release_year 2023
title Alpha-synuclein oligomers alter the spontaneous firing discharge of cultured midbrain neurons
volume 17
webcaptures []
work_id wuoat3hyjncbfmewp5chbk7de4

Extra Metadata (raw JSON)

crossref.alternative-id ['10.3389/fncel.2023.1078550']
crossref.license [{'URL': '', 'content-version': 'vor', 'delay-in-days': 0, 'start': '2023-01-20T00:00:00Z'}]
crossref.subject ['Cellular and Molecular Neuroscience']
crossref.type journal-article
pubmed.pub_types ['Journal Article']