Down-regulated gene expression spectrum and immune responses changed during the disease progression in COVID-19 patients
release_e72vjipe5bafjduladpgnmuwuy
by
Yabo Ouyang, Jiming Yin, Wenjing Wang, Hongbo Shi, Ying Shi, Bin Xu, Luxin Qiao, Yingmei Feng, Lijun Pang, Feili Wei, Xianghua Guo, Ronghua Jin (+1 others)
2020 Volume 71, Issue 16, p2052-2060
Abstract
<jats:title>Abstract</jats:title>
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<jats:title>Background</jats:title>
WHO characterizes novel coronavirus disease (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as a pandemic. Here, we investigated the clinical, cytokine levels, T cell proportion and related gene expression occurring in COVID-19 patients on admission and after intial treatment.
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<jats:title>Methods</jats:title>
11 patients diagnosed as COVID-19 with similar initial treatment regimen were enrolled in the hospital. Plasma cytokines, CyTOF and microfluidic qPCR for gene expression were conducted.
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<jats:title>Results</jats:title>
5 mild and 6 severe patients were included. Cough and fever were the top symptoms in the 11 COVID-2019 cases. The elder age, more neutrophils numbers and higher C-reactive protein level were found in severe cases. IL-10 level was significantly varied with disease progression and treatment. The decreased T cell proportions were observed in COVID-19 patients especially in severe cases, and all elevated to normal in mild patiens after initial treatment but only CD4+T cells return to normal in severe cases. The number of DEGs increased with the disease progress, and decreased after initial treatment. All down-regulated DEGs in severe cases mainly involved in Th17 cell differentiation, cytokine-mediated signaling pathway and T cell activation. After initial treatmen in severe cases, MAP2K7 and SOS1 were upregulated relative to that on admission.
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<jats:title>Conclusions</jats:title>
Our findings show a decreased T cell proportion with down-regulated gene expression related to T cell activation and differentiation were occurred in COVID-19 severe patients, which may help to provide effective treatment strategies for COVID-19 .
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