Evaluation of Antioxidant Capacity and Antiproliferative Activity of Fruit Extract of Dry Figs (Ficus carica L.) on MDA MB-468 Cell Line release_d23zvzg2ovauvhruomzwk3ebxy

by Anurag Maurya, Eepsita Priyadarshini, Paulraj Rajamani

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<jats:title>Abstract</jats:title> Fig (<jats:italic>Ficus carica </jats:italic>L.; Hindi: Anjeer) is one of the the earliest domesticated fruit. Present study investigates its antiproliferative activity against breast cancer (MDA MB-468) cells. Dry figs were mascerated in sixteen solvent-combinations of varying polartiy and extracts were assessed for antiproliferative activity <jats:italic>in vitro</jats:italic>. Extracts of moderatly polar solvents, particularly 80% aqueous-methanol extract showed maximum activity (IC<jats:sub>50 </jats:sub>= 17.9 mg IDF/ml). Antiproliferative efficacy of extracts was supported by respective total phenolic content (41-236 mg GAE/100g) and various antioxidant capacities; ABTS (14-354 µM TE/g), DPPH (18-460 µM TE/g), FRAP (28.7-66.5 µM FeSO<jats:sub>4</jats:sub> equivalents/g), ORAC (7.9-32.8 µM TE/g), and superoxide radical scavenging activity. Flow cytometry analysis revealed that extracts of lower IC<jats:sub>50</jats:sub> induce more cell death. Phenolic compounds (cyanidin 3-O-glucoside, quercetin 3-β-glucoside, rutin, trans-cinnamic acid, ellagic acid and ferulic acid) were analysed by UPLC-PDA technique and their concentrations in extracts were found to be negatively correlated with respective IC<jats:sub>50 </jats:sub>values. The negative correlation suggests the involvement of phenolics in fig's antiproliferative mechanism. Therefore, the study advocates fig as a dietary component having anticancer potential.
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