LncRNA LINC00240 suppresses invasion and migration in non-small cell lung cancer by sponging miR-7-5p release_cu5tafutg5ajtcib2pfilhcbya

by gwan woo Ku, Yujin Kang, Seong-Lan Yu, Joonghoon Park, Sejin Park, In Beom Jeong, Min Woong Kang, Ji Woong Son, Jaeku Kang

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<jats:title>Abstract</jats:title> Background lncRNAs have important roles in regulating cancer biology. Accumulating evidence has established a link between the dysregulation of lncRNAs and microRNA in cancer progression. In previous studies, <jats:italic>miR-7-5p</jats:italic> has been found to be significantly down-regulated in mesenchymal-like lung cancer cell lines and directly regulated <jats:italic>EGFR</jats:italic>. In this work, we investigated the lncRNA partner of <jats:italic>miR-7-5p</jats:italic> in the progression of lung cancer. Methods We investigated the expression of <jats:italic>miR-7-5p</jats:italic> and the lncRNA after transfection with an <jats:italic>miR-7-5p</jats:italic> mimics using a microarray. The microarray results were validated using quantitative real time-polymerase Chain Reaction (qRT-PCR). The regulatory effects of lncRNA on <jats:italic>miR-7-5p</jats:italic> and its target were evaluated by changes in the expression of <jats:italic>miR-7-5p</jats:italic> after transfection with siRNAs for lncRNA and the synthesis of full-length lncRNA. The effect of <jats:italic>miR-7-5p</jats:italic> on lncRNA and the miRNA target was evaluated after transfection with miRNA mimic and inhibitor. The role of lncRNA in cancer progression was determined using invasion and migration assays. The level of lncRNA in lung cancer and normal lung tissue was analyzed using TCGA data. Results We found that <jats:italic>LINC00240</jats:italic> was downregulated in lung cancer cell line after <jats:italic>miR-7-5p</jats:italic> trandfection with an <jats:italic>miR-7-5p</jats:italic> mimic. Further investigations reveal ed that the knockdown of <jats:italic>LINC00240</jats:italic> induced the overexpression of <jats:italic>miR-7-5p</jats:italic>. The overexpression of <jats:italic>miR-7-5p</jats:italic> diminished cancer invasion and migration. The <jats:italic>EGFR</jats:italic> expression was down regulated after siRNA treatment for <jats:italic>LINC00240</jats:italic>. Silencing <jats:italic>LINC00240</jats:italic> suppressed the invasion and migration of lung cancer cells, whereas <jats:italic>LINC00240</jats:italic> overexpression exerted the opposite effect. The lower expression of <jats:italic>LINC00240</jats:italic> in squamous lung cancer was analyzed using TCGA data. Conclusions Taken together, <jats:italic>LINC00240</jats:italic> acted as a sponge for <jats:italic>miR-7-5p</jats:italic> and induced the overexpression of <jats:italic>EGFR</jats:italic>. <jats:italic>LINC00240</jats:italic> may represent a potential target for the treatment of lung cancer.
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