MOESM1 of Metabolic labeling in middle-down proteomics allows for investigation of the dynamics of the histone code release_cha77msbd5ghlcuxwbkviipwma

Abstract

Additional file 1: Figure S1. Analysis of EMT cells labeled using heavy lysine/arginine (heavy KR) during mesenchymal transition. (A) Workflow for metabolic labeling of EMT cells at the histone sequence level (heavy KR). (B) Pearson's correlation values between technical and biological replicates (rep 2-1 implies second biological, first technical), differentiated between combinatorial PTMs quantified on unlabeled histone tails (old histones) and heavy labeled histone tails (new). (C) Bar plot representing the relative abundance of single histone PTMs on heavy labeled histone tails at day 1 (top) and day 2 (bottom). Unlabeled histone tails were excessively low abundant to provide a confident quantification of single histone PTMs. Figure S2. Annotated MS/MS spectrum of the histone H3 N-terminal tail modified as K27me2. From the top to bottom, (i) annotated sequence with highlights of identified fragments and modified site, (ii) annotated spectrum, (iii) observed mass, identified modification and Mascot (Matrix Science) identification scores, (iv) distribution of the fragment mass error, expressed in Da (left) and ppm (right). Figure S3. Annotated MS/MS spectrum of the histone H3 N-terminal tail modified as K27me2:2. From the top to bottom, (i) annotated sequence with highlights of identified fragments and modified site, (ii) annotated spectrum, (iii) observed mass, identified modification and Mascot (Matrix Science) identification scores, (iv) distribution of the fragment mass error, expressed in Da (left) and ppm (right). Figure S4. Annotated MS/MS spectrum of the histone H3 N-terminal tail modified as K14acK27me2:2. From the top to bottom, (i) annotated sequence with highlights of identified fragments and modified site, (ii) annotated spectrum, (iii) observed mass, identified modification and Mascot (Matrix Science) identification scores, (iv) distribution of the fragment mass error, expressed in Da (left) and ppm (right). Figure S5. Annotated MS/MS spectrum of the histone H3 N-terminal tail modified as K23me1:1 [...]
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