Imaging Hallmarks of the Tumor Microenvironment in Glioblastoma Progression release_bzx2tdytsfarvbaakq7gpt2zsy

by John J. Walsh, Maxime Parent, Adil Akif, Lucas C. Adam, Samuel Maritim, Sandeep K. Mishra, Muhammad H. Khan, Daniel Coman, Fahmeed Hyder

Published in Frontiers in Oncology by Frontiers Media SA.

2021   Volume 11, p692650

Abstract

Glioblastoma progression involves multifaceted changes in vascularity, cellularity, and metabolism. Capturing such complexities of the tumor niche, from the tumor core to the periphery, by magnetic resonance imaging (MRI) and spectroscopic imaging (MRSI) methods has translational impact. In human-derived glioblastoma models (U87, U251) we made simultaneous and longitudinal measurements of tumor perfusion (F<jats:sub>p</jats:sub>), permeability (K<jats:sup>trans</jats:sup>), and volume fractions of extracellular (v<jats:sub>e</jats:sub>) and blood (v<jats:sub>p</jats:sub>) spaces from dynamic contrast enhanced (DCE) MRI, cellularity from apparent diffusion coefficient (ADC) MRI, and extracellular pH (pH<jats:sub>e</jats:sub>) from an MRSI method called Biosensor Imaging of Redundant Deviation in Shifts (BIRDS). Spatiotemporal patterns of these parameters during tumorigenesis were unique for each tumor. While U87 tumors grew faster, F<jats:sub>p</jats:sub>, K<jats:sup>trans</jats:sup>, and v<jats:sub>p</jats:sub> increased with tumor growth in both tumors but these trends were more pronounced for U251 tumors. Perfused regions between tumor periphery and core with U87 tumors exhibited higher F<jats:sub>p</jats:sub>, but K<jats:sup>trans</jats:sup> of U251 tumors remained lowest at the tumor margin, suggesting primitive vascularization. Tumor growth was uncorrelated with v<jats:sub>e</jats:sub>, ADC, and pH<jats:sub>e</jats:sub>. U87 tumors showed correlated regions of reduced v<jats:sub>e</jats:sub> and lower ADC (higher cellularity), suggesting ongoing proliferation. U251 tumors revealed that the tumor core had higher v<jats:sub>e</jats:sub> and elevated ADC (lower cellularity), suggesting necrosis development. The entire tumor was uniformly acidic (pH<jats:sub>e</jats:sub> 6.1-6.8) early and throughout progression, but U251 tumors were more acidic, suggesting lower aerobic glycolysis in U87 tumors. Characterizing these cancer hallmarks with DCE-MRI, ADC-MRI, and BIRDS-MRSI will be useful for exploring tumorigenesis as well as timely therapies targeted to specific vascular and metabolic aspects of the tumor microenvironment.
In application/xml+jats format

Archived Files and Locations

application/pdf  5.5 MB
file_25qhpafhszgqtd3mot7ab4ltbi
fjfsdata01prod.blob.core.windows.net (web)
web.archive.org (webarchive)

Web Captures

https://www.frontiersin.org/articles/10.3389/fonc.2021.692650/full
2022-08-07 15:03:21 | 33 resources
webcapture_nadsnvcmfngwzlgjvyn4udwnai
web.archive.org (webarchive)
Read Archived PDF
Preserved and Accessible
Type  article-journal
Stage   published
Date   2021-08-26
Language   en ?
Container Metadata
Open Access Publication
In DOAJ
In ISSN ROAD
In Keepers Registry
ISSN-L:  2234-943X
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: 319b941a-0cfc-41f8-a995-ea2a1c73c136
API URL: JSON