Discrimination of Stressed and Non-Stressed Food-Related Bacteria Using Raman-Microspectroscopy release_ahqpnbmtfbbstfv4iwusxpoewa

by Daniel Klein, Rene Breuch, Jessica Reinmüller, Carsten Engelhard, Peter Kaul

Published in Foods by MDPI AG.

2022   Volume 11, Issue 10, p1506

Abstract

As the identification of microorganisms becomes more significant in industry, so does the utilization of microspectroscopy and the development of effective chemometric models for data analysis and classification. Since only microorganisms cultivated under laboratory conditions can be identified, but they are exposed to a variety of stress factors, such as temperature differences, there is a demand for a method that can take these stress factors and the associated reactions of the bacteria into account. Therefore, bacterial stress reactions to lifetime conditions (regular treatment, 25 °C, HCl, 2-propanol, NaOH) and sampling conditions (cold sampling, desiccation, heat drying) were induced to explore the effects on Raman spectra in order to improve the chemometric models. As a result, in this study nine food-relevant bacteria were exposed to seven stress conditions in addition to routine cultivation as a control. Spectral alterations in lipids, polysaccharides, nucleic acids, and proteins were observed when compared to normal growth circumstances without stresses. Regardless of the involvement of several stress factors and storage times, a model for differentiating the analyzed microorganisms from genus down to strain level was developed. Classification of the independent training dataset at genus and species level for Escherichia coli and at strain level for the other food relevant microorganisms showed a classification rate of 97.6%.
In application/xml+jats format

Archived Files and Locations

application/pdf  1.4 MB
file_wk255zn7ljbk5oz76noc3uxrma
mdpi-res.com (publisher)
web.archive.org (webarchive)

Web Captures

https://www.mdpi.com/2304-8158/11/10/1506/htm
2022-06-23 12:11:08 | 43 resources
webcapture_ojlasll2ejgmljc2bwgliuqmpi
web.archive.org (webarchive)
Read Archived PDF
Preserved and Accessible
Type  article-journal
Stage   published
Date   2022-05-22
Language   en ?
Container Metadata
Open Access Publication
In DOAJ
In ISSN ROAD
In Keepers Registry
ISSN-L:  2304-8158
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: 69c9aa1c-cec4-4052-a7d0-9d3d208a6976
API URL: JSON