Long Non-Coding RNA Deregulation in Tongue Squamous Cell Carcinoma release_acwgo2zvmbbv7b7nsczhrtkniy

by Wei Gao, Jimmy Yu-Wai Chan, Thian-Sze Wong

Published in BioMed Research International by Hindawi Limited .

Volume 2014p1-10 (2014)

Abstract

<jats:italic>Background.</jats:italic>The deregulated tumorigenic long non-coding RNA (lncRNA) has been reported in several malignancies. However, there is still no comprehensive study on tongue squamous cell carcinoma (SCC).<jats:italic>Methods.</jats:italic>Functional reannotation for the human lncRNA was carried out by ncFANs. Real-time quantitative PCR was used to validate the identified lncRNAs.<jats:italic>Results.</jats:italic>Using the functional annotation algorithm from ncFANs, 8 differentially expressed lncRNAs were identified. Lnc-PPP2R4-5, lnc-SPRR2D-1, lnc-MAN1A2-1, lnc-FAM46A-1, lnc-MBL2-4:1, and lnc-MBL2-4:3 were upregulated in the microdissected tongue SCC tissues. In comparison, lnc-AL355149.1-1 and lnc-STXBP5-1 showed significant downregulation. High level of lnc-MBL2-4:3 was significantly associated with the node positive tongue SCC patients. Further, patients with advanced T-stage demonstrated a further reduction of lnc-AL355149.1-1 in the tumor tissues. Treatment of tongue SCC cells with 5-fluorouracil and paclitaxel can reserve the expression patterns observed in the tongue SCC tissues. Further, changes of lnc-MBL2-4:3 and lnc-AL355149.1-1 expression levels were noticed in the cisplatin-resistant tongue SCC cells.<jats:italic>Conclusions.</jats:italic>Our results demonstrated that functional reannotation allows us to identify novel lncRNAs using the existing gene expression array dataset. The association of lncRNA with the T-stage and nodal status of tongue SCC patients suggested that lncRNA deregulation was involved in the pathogenesis of tongue SCC.
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Type  article-journal
Stage   published
Year   2014
Language   en ?
DOI  10.1155/2014/405860
PubMed  25045670
PMC  PMC4090519
Wikidata  Q33876539
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