Viability of Escherichia coli ATCC 8739 in Nutrient Broth, Luria-Bertani Broth and Brain Heart Infusion over 11 Weeks release_7lkmn6ef55dhpow6yvvkesgxtq

by Samuel Xin Zher Low, Zhen Qin Aw, Bryan Zong Lin Loo, Kun Cheng Lee, Jack Si Hao Oon, Chin How Lee, Maurice Han Tong Ling

Published in Electronic Physician .

Volume 5, Issue 1 p576-81 (2013)

Abstract

Escherichia coli is a widely studied prokaryotic system. A recent study had demonstrated that reduced growth of E. coli after extended culture in Luria-Bertani broth is a result of depletion of fermentable sugars but able to sustain extended cell culture due to the presence of amino acids, which can be utilized as a carbon source. However, this had not been demonstrated in other media. The study aimed to determine the growth and viability of E. coli ATCC 8739 in 3 different media, Nutrient Broth (NB), Brain Heart Infusion (BHI) and Luria-Bertani Broth (LB) over 11 weeks. Growth of E. coli ATCC 8739 was determined by optical density. Viability was determined by serial dilution/spread-plate enumeration. After 11 weeks, the media were exhausted by repeated culture. Glucose was added to the exhausted media to determine whether glucose is the growth-limiting factor. Our results showed that cell density in all 3 media increased to about 1 × 10(9) cells/ml by the end of week 1, from the inoculation density of 2.67 × 10(5) cells/ml, peaked at about 1 × 10(13) cells/ml at week 4, before declining to about 5 × 10(7) cells/ml at week 7. Cell density is highly correlated to genomic DNA content (r(2) = 0.93) but poorly correlated to optical density (r(2)< 0.2). Our results also showed that the spent media were able to support further growth after glucose-supplementation. NB, LB and BHI are able to support extended periods of culture and glucose depletion is the likely reason for declining cell growth.
In text/plain format

Archived Files and Locations

application/pdf  492.7 kB
file_gxrdddwikbhylipqrfprwsxw3m
europepmc.org (repository)
web.archive.org (webarchive)
Read Archived PDF
Archived
Type  article-journal
Stage   published
Date   2013-02-01
Language   en ?
Container Metadata
Open Access Publication
In DOAJ
In ISSN ROAD
Not in Keepers Registry
ISSN-L:  2008-5842
Work Entity
access all versions, variants, and formats of this works (eg, pre-prints)
Catalog Record
Revision: ab27390c-2163-4859-ab2c-b10dd67253d8
API URL: JSON