A PCR-based NGS protocol for whole genome sequencing of West Nile virus lineage 2 directly from biological specimens release_66nj5ox2bja5teluzbmib3oemm

by Serafeim C. Chaintoutis, Elpida Papadopoulou, Angeliki Melidou, Anna Papa, Chrysostomos I. Dovas

Abstract

Lineage 2 West Nile virus (WNV) strains have been implicated in severe encephalitis outbreaks in humans and equines residing in Europe. WNV molecular characterization is important for the development of diagnostic assays, as well as for obtaining molecular information, which is necessary for epidemiological investigations of virus transmission in areas at risk. For whole genome sequencing of lineage 2 WNV strains, directly from biological specimens, a PCR-based NGS protocol was developed. The method was applied in WNV-positive specimens obtained from animal, human and mosquito hosts in Greece. The results of its application indicate that, even in cases of low virus titers, the developed PCR-based NGS approach is able to provide whole genome sequences of lineage 2 WNV strains.
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Published in Molecular and Cellular Probes by Elsevier BV
ISSN-L 0890-8508
Volume 46
Page(s) 101412
Release Date 2019-06-12
Publisher Elsevier BV
Primary Language en (lookup)

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Type  article-journal
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Date   2019-06-12
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ISSN-L:  0890-8508
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