optoPAD: a closed-loop optogenetics system to study the circuit basis of feeding behaviors
release_5g2bu6x3rzcu5ick7cw3wpmbqm
by
José-Maria Moreira, Pavel Itskov, Dennis Goldschmidt, Kathrin Steck, Samuel Walker, Carlos Ribeiro
2018
Abstract
The regulation of feeding plays a key role in determining the fitness of animals through its impact on nutrition. Elucidating the circuit basis of feeding and related behaviors is an important goal in neuroscience. We recently used a system for closed-loop optogenetic manipulation of neurons contingent on the fly's feeding behavior to dissect the impact of a specific subset of taste neurons on yeast feeding (Steck et al., 2018). Here we describe the development and validation of this system, which we term the optoPAD. We use the optoPAD to induce appetitive and aversive effects on feeding by activating or inhibiting gustatory neurons in closed loop – effectively creating virtual taste realities. The use of optogenetics allowed us to vary the dynamics and probability of stimulation in single flies and assess the impact on feeding behavior quantitatively and with high throughput. These data demonstrate that the optoPAD is a powerful tool to dissect the circuit basis of feeding behavior, allowing the efficient implementation of sophisticated behavioral paradigms to study the mechanistic basis of animals' adaptation to dynamic environments.
In application/xml+jats
format
Archived Files and Locations
application/pdf 5.9 MB
file_t6rxz6l6ojh25apalrawpan5gu
|
www.biorxiv.org (web) web.archive.org (webarchive) |
post
Stage
unknown
Date 2018-12-03
10.1101/485110
access all versions, variants, and formats of this works (eg, pre-prints)
Crossref Metadata (via API)
Worldcat
wikidata.org
CORE.ac.uk
Semantic Scholar
Google Scholar