Changes in Gene Expression during Larval Development of Calliphora vicina Induced by Ecdysterone
release_3c2tjr72nngl7oj6phmbjtsgl4
by
Peter Mascheck, Klaus Scheller, Peter Karlson
1977 Volume 32, Issue 5-6, p434-438
Abstract
<jats:title>Abstract</jats:title>
The influence of the insect hormone ecdysterone on the composition of the RNA from the fat bodies of Calliphora larvae was investigated by hybridization experiments. DNA was iodinated in vitro with <jats:sup>125</jats:sup>J. In saturation experiments, total RNA was hybridized to total DNA; under similar conditions, poly (A) RNA was hybridized to unique DNA sequences. RNA isolated from animals treated with ecdysterone exhibits a higher percentage of hybridization than a corresponding control RNA from the same developmental stage, a result even more prominent when poly (A) RNA was hybridized with unique DNA. It is concluded that ecdysterone induces the transcription of new unique DNA sequences.
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Date 1977-06-01
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