Comprehensive analysis of human hookworm secreted proteins using a proteogenomic approach release_24mu2zx3qzc3jpuaoziobvvsje

by Jayden Logan, Srinivas Srikanth Manda, Young-Jun Choi, Matthew Field, Ramon Eichenberger, Jason Mulvenna, Shivashankar Nagaraj, Ricardo Fujiwara, Pedro Gazzinelli-Guimaraes, Lilian Bueno, Vitor Mati, Makedonka Mitreva (+2 others)

Released as a post by Cold Spring Harbor Laboratory.

2018  

Abstract

The human hookworm Necator americanus infects more than 400 million people worldwide, contributing substantially to the poverty in these regions. Adult stage N. americanus live in the small intestine of the human host where they inject excretory/secretory (ES) products into the mucosa. ES products have been characterized at the proteome level for a number of animal hookworm species, but until now, the difficulty in obtaining sufficient live N. americanus has been an obstacle in characterizing the secretome of this important human pathogen. Herein we describe the ES proteome of N. americanus and utilize this information to conduct the first proteogenomic analysis of a parasitic helminth, significantly improving the available genome and thereby generating a robust description of the parasite secretome. The genome annotation resulted in a a revised prediction of 3,425 fewer genes than initially reported, accompanied by a significant increase in the number of exons and introns, total gene length and the percentage of the genome covered by genes. Almost 200 ES proteins were identified by LC-MS/MS with SCP/TAPS proteins, "hypothetical" proteins and proteases among the most abundant families. These proteins were compared to commonly used model species of human parasitic infections, including Ancylostoma caninum, Nippostrongylus brasiliensis and Heligmosomoides polygyrus. Our findings provide valuable information on important families of proteins with both known and unknown functions that could be instrumental in host-parasite interactions, including protein families that might be key for parasite survival in the onslaught of robust immune responses, as well as vaccine and drug targets.
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Date   2018-09-03
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