Immune gene expression in gilthead seabream ( Sparus aurata ) after Lymphocystis disease virus (LCDV‐Sa) challenge resulting in asymptomatic infection release_bs4seg6xljet7i7gwldq7adulu [as of editgroup_nc46ur67gzcnfdychzxtp5ldji]

by R. Leiva‐Rebollo, A.M. Labella, J.J. Borrego, D. Castro

Published in Journal of Applied Microbiology by Wiley.

2019   Volume 128, Issue 1, p41-53

Abstract

To determine the immune gene expression response of gilthead seabream (Sparus aurata) that is experimentally infected with the lymphocystivirus LCDV-Sa. Viral DNA and transcripts were detected by qPCR in all samples from fish injected with LCDV-Sa, demonstrating that the virus establish a systemic and asymptomatic infection. The expression of 23 immune-related genes was also analysed by RT-qPCR in the head kidney (HK) and intestine at several times post-infection (dpi). In HK, the expression of five type I interferon (IFN)-related genes (ifn, irf3, mx2, mx3 and isg15), il10 and ck10 was upregulated at 1-3 dpi, while genes related to the inflammation process (tnfα, il1ß, il6, casp1) were not differentially expressed or even downregulated. The expression profile in the intestine was different regarding type I INF-related genes. An upregulated c3 and ighm expression was observed in both HK and intestine at 3-8 dpi. Finally, the transcription of nccrp1 and mhcIIα was induced in HK, whereas tcrβ expression was downregulated in both organs. LCDV-Sa seems to trigger an immune response in gilthead seabream characterized by a partial activation of type I IFN system and a lack of systemic inflammatory response which may be related to viral persistence. The immune response observed in gilthead seabream infected by LCDV-Sa could be implicated in the establishment of an asymptomatic persistent infection.
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Type  article-journal
Stage   published
Date   2019-09-17
Language   en ?
DOI  10.1111/jam.14454
PubMed  31529740
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ISSN-L:  1364-5072
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