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Revision: b494986f-1851-4ef0-a20e-48977289f3b9
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+content = "<jats:sec id=\"sec001\">\n<jats:title>Objectives</jats:title>\n<jats:p>To examine Medicare health care spending and health services utilization among high-need population segments in older Mexican Americans, and to examine the association of frailty on health care spending and utilization.</jats:p>\n</jats:sec>\n<jats:sec id=\"sec002\">\n<jats:title>Methods</jats:title>\n<jats:p>Retrospective cohort study of the innovative linkage of Medicare data with the Hispanic Established Populations for the Epidemiologic Study of the Elderly (H-EPESE) were used. There were 863 participants, which contributed 1,629 person years of information. Frailty, cognition, and social risk factors were identified from the H-EPESE, and chronic conditions were identified from the Medicare file. The Cost and Use file was used to calculate four categories of Medicare spending on: hospital services, physician services, post-acute care services, and other services. Generalized estimating equations (GEE) with a log link gamma distribution and first order autoregressive, correlation matrix was used to estimate cost ratios (CR) of population segments, and GEE with a logit link binomial distribution was applied to estimate odds ratios (OR) of healthcare use.</jats:p>\n</jats:sec>\n<jats:sec id=\"sec003\">\n<jats:title>Results</jats:title>\n<jats:p>Participants in the major complex chronic illness segment who were also pre-frail or frail had higher total costs and utilization compared to the healthy segment. The CR for total Medicare spending was 3.05 (95% CI, 2.48–3.75). Similarly, this group had higher odds of being classified in the high-cost category 5.86 (95% CI, 3.35–10.25), nursing home care utilization 11.32 (95% CI, 3.88–33.02), hospitalizations 4.12 (95% CI, 2.88–5.90) and emergency room admissions 4.24 (95% CI, 3.04–5.91).</jats:p>\n</jats:sec>\n<jats:sec id=\"sec004\">\n<jats:title>Discussion</jats:title>\n<jats:p>Our findings highlight that frailty assessment is an important consideration when identifying high-need and high-cost patients.</jats:p>\n</jats:sec>"
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+[[refs]]
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+container_name = "J Archaeol Sci"
+title = "Wound ballistics: The prey specific implications of penetrating trauma injuries from osseous, flaked stone, and composite inset microblade projectiles during the Pleistocene/Holocene transition, Alaska U.S.A"
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+year = 2006
+container_name = "Towar a Defin Aurignacian Proc Symp held Lisbon, Port June 25–30, 2002"
+title = "A brief overview of Aurignacian cultures in the context of the industries of the transition from the Middle to the Upper Paleolithic"
+locator = "133"
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+[[refs]]
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+year = 2013
+container_name = "Les traces de notre passé européen. Le Protoaurignacien au début du Paléolithique supérieur: l'éclairage de la tracéologie"
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+year = 2010
+container_name = "Gall Préhistoire"
+title = "Identité technique et comportements économiques des groupes proto-aurignaciens à la grotte de l'Observatoire (principauté de Monaco)."
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+
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+[[refs]]
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+year = 2017
+container_name = "Open Archaeol."
+title = "Hunting high and low: Gravettian hunting weapons from Southern Italy to the Russian Plain"
+locator = "376"
+
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+doi = "10.1515/opar-2017-0024"
+[[refs]]
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+key = "pone.0262185.ref145"
+year = 2013
+container_name = "J Archaeol Sci"
+title = "Evidence from the Yana Palaeolithic site, Arctic Siberia, yields clues to the riddle of mammoth hunting"
+locator = "4189"
+
+[refs.extra]
+authors = [ "P Nikolskiy",]
+doi = "10.1016/j.jas.2013.05.020"
+volume = "40"
+[[refs]]
+index = 145
+key = "pone.0262185.ref146"
+year = 1989
+container_name = "Bull la Société préhistorique française"
+title = "L'utilisation des microlithes géométriques et non géométriques comme armatures de projectiles"
+locator = "88"
+
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+doi = "10.3406/bspf.1989.9368"
+volume = "86"
+[[refs]]
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+year = 2006
+container_name = "Archaeol Ethnol Anthropol Eurasia"
+title = "Lugovskoe: Geoarchaeology and culture of a Paleolithic site"
+locator = "41"
+
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+volume = "25"
+[[refs]]
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+year = 1982
+container_name = "Archäologisches Korrespondenzblatt"
+title = "Zur Jagd im Jungpaläolithikum: Nach Beispielen des jungpaläolithischen Fundplatzes Kokorevo I in Sibirien"
+
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+volume = "12"
+[[refs]]
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+key = "pone.0262185.ref149"
+year = 1983
+container_name = "Bull la Société préhistorique française."
+title = "Une tête de sagaie à armature de lamelles de silex à Pincevent (Seine-et-Marne)"
+locator = "154"
+
+[refs.extra]
+authors = [ "A. Leroi-Gourhan",]
+doi = "10.3406/bspf.1983.5435"
+volume = "80"
+[[refs]]
+index = 149
+key = "pone.0262185.ref150"
+year = 1984
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+title = "The Paleohistory of Circumpolar Arctic Colonization"
+locator = "359"
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+doi = "10.14430/arctic2220"
+volume = "37"
+[[refs]]
+index = 150
+key = "pone.0262185.ref151"
+year = 2018
+container_name = "J Archaeol Sci"
+title = "A techno-functional perspective on quartz micro-notches in Sibudu's Howiesons Poort reveals the use of barbs in hunting technology"
+locator = "166"
+
+[refs.extra]
+authors = [ "P de la Peña",]
+doi = "10.1016/j.jas.2018.03.001"
+volume = "93"
+[[abstracts]]
+sha1 = "1f0a78bee5781c30a1dee2e7dd52d482fe63aed0"
+content = "<jats:p>Projectile technology is commonly viewed as a significant contributor to past human subsistence and, consequently, to our evolution. Due to the allegedly central role of projectile weapons in the food-getting strategies of Upper Palaeolithic people, typo-technological changes in the European lithic record have often been linked to supposed developments in hunting weaponry. Yet, relatively little reliable functional data is currently available that would aid the detailed reconstruction of past weapon designs. In this paper, we take a use-wear approach to the backed tool assemblages from the Recent and Final Gravettian layers (Levels 3 and 2) of Abri Pataud (Dordogne, France). Our use of strict projectile identification criteria relying on combinations of low and high magnification features and our critical view of the overlap between production and use-related fractures permitted us to confidently identify a large number of used armatures in both collections. By isolating lithic projectiles with the strongest evidence of impact and by recording wear attributes on them in detail, we could establish that the hunting equipment used during the Level 3 occupations involved both lithic weapon tips and composite points armed with lithic inserts. By contrast, the Level 2 assemblage reflects a heavy reliance on composite points in hunting reindeer and other game. Instead of an entirely new weapon design, the Level 2 collection therefore marks a shift in weapon preferences. Using recent faunal data, we discuss the significance of the observed diachronic change from the point of view of prey choice, seasonality, and social organisation of hunting activities. Our analysis shows that to understand their behavioural significance, typo-technological changes in the lithic record must be viewed in the light of functional data and detailed contextual information.</jats:p>"
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+content = "<p>Mass releases of two parasitoid species, Aphidius matricariae and Ephedrus cerasicola, may provide an alternative measure to pesticides to control the rosy apple aphid Dysaphis plantaginea in organic apple orchards. As a proof of concept study, we tested if the presence of flower strips between apple tree rows could improve the action of three early parasitoid releases—and of other naturally present aphid enemies—on the control of aphid colonies and number of aphids per tree. Apple trees located at different distances from parasitoid release points were monitored in plots with and without flower strips in an organic apple orchard over two years, along the season of aphid infestation (March to July). Our case study demonstrated that the presence of flowering plant mixes in the alleyways of an apple orchard improved the biological control of D. plantaginea, with an effect size of 33.4% less aphids in plots with flower strips, compared to plots without flower strips, at the infestation peak date. We also showed a negative effect of higher distance to parasitoid release points on aphid control, but our results at the infestation peak date suggest that the presence of flowers could marginally compensate for the detrimental effect of distance, probably by improving the persistence and dispersal capacities of natural enemies. Despite high variations in aphid population dynamics between years, we conclude that combining flower strips with early parasitoid releases in apple orchards is promising for biological control of the rosy apple aphid, although the method merits to be further refined and repeated in more orchards.</p>"
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Revision: 2f0dabb1-0c49-4b1d-8e37-2fc0d41dfbc0
--- (created)
+++ /release/rev/2f0dabb1-0c49-4b1d-8e37-2fc0d41dfbc0.toml
@@ -0,0 +1,98 @@
+title = "Single-particle fluorescence tracking combined with TrackMate assay reveals highly heterogeneous and discontinuous lysosomal transport in freely orientated axons"
+work_id = "fh3ldxvr7vhvzimjjnvmo7gq6q"
+release_type = "post"
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+release_year = 2022
+publisher = "Authorea, Inc."
+refs = []
+[[contribs]]
+index = 0
+raw_name = "Yongyang Liu"
+given_name = "Yongyang"
+surname = "Liu"
+role = "author"
+raw_affiliation = "University of Science and Technology of China"
+
+[contribs.extra]
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+[[contribs]]
+index = 1
+raw_name = "Yaxin Lu"
+given_name = "Yaxin"
+surname = "Lu"
+role = "author"
+raw_affiliation = "University of Science and Technology of China"
+
+[[contribs]]
+index = 2
+raw_name = "Zhiyong Tang"
+given_name = "Zhiyong"
+surname = "Tang"
+role = "author"
+raw_affiliation = "University of Science and Technology of China"
+
+[[contribs]]
+index = 3
+raw_name = "Yuheng Cao"
+given_name = "Yuheng"
+surname = "Cao"
+role = "author"
+raw_affiliation = "ShanghaiTech University"
+
+[[contribs]]
+index = 4
+raw_name = "Dehua Huang"
+given_name = "Dehua"
+surname = "Huang"
+role = "author"
+raw_affiliation = "Suzhou Institute of Nano-tech and Nano-Bionics Chinese Academy of Sciences"
+
+[[contribs]]
+index = 5
+raw_name = "Feng Wu"
+given_name = "Feng"
+surname = "Wu"
+role = "author"
+raw_affiliation = "Suzhou Institute of Nano-tech and Nano-Bionics Chinese Academy of Sciences"
+
+[[contribs]]
+index = 6
+raw_name = "Yejun Zhang"
+given_name = "Yejun"
+surname = "Zhang"
+role = "author"
+raw_affiliation = "Suzhou Institute of Nano-tech and Nano-Bionics Chinese Academy of Sciences"
+
+[[contribs]]
+index = 7
+raw_name = "Chunyan Li"
+given_name = "Chunyan"
+surname = "Li"
+role = "author"
+raw_affiliation = "Suzhou Institute of Nano-tech and Nano-bionics"
+
+[[contribs]]
+index = 8
+raw_name = "Guangcun Chen"
+given_name = "Guangcun"
+surname = "Chen"
+role = "author"
+raw_affiliation = "Suzhou Institute of Nano-tech and Nano-bionics"
+
+[[contribs]]
+index = 9
+raw_name = "Qiangbin Wang"
+given_name = "Qiangbin"
+surname = "Wang"
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+content = "<jats:p id=\"p1\">Axonal transport plays a significant role in the establishment of\nneuronal polarity, axon growth, and synapse formation during neuronal\ndevelopment. The axon of a naturally growing neuron is a highly complex\nand multifurcated structure with a large number of bends and branches.\nNowadays, the study of dynamic axonal transport in morphologically\ncomplex neurons is greatly limited by the technological barrier. Here, a\nsparse gene transfection strategy was developed to locate fluorescent\nmCherry in the lysosome of primary neurons, thus enabling us to track\nthe lysosome-based axonal transport with a single-particle resolution.\nThereby, several axonal transport models were observed, including\nforward or backward transport model, stop-and-go model, repeated\nback-and-forth transport model, and cross-branch transport model. Then,\nthe accurate single-particle velocity quantification by TrackMate\nrevealed a highly heterogeneous and discontinuous transportation process\nof lysosome-based axonal transport in freely orientated axons. And,\nmultiple physical factors, such as the axonal structure and the size of\nparticles, were disclosed to affect the velocity of particle\ntransporting in freely orientated axons. The combined single-particle\nfluorescence tracking and TrackMate assay can be served as a facile tool\nfor evaluating axonal transport in neuronal development and axonal\ntransport-related diseases.</jats:p>"
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+
+[ext_ids]
+doi = "10.22541/au.164219078.86486130/v1"
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+[extra.crossref]
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Revision: 1bf30963-db26-47aa-a35e-0e43a70a0f73
--- (created)
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+release_date = "2021-12-21"
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+pages = "V-VI"
+publisher = "Presses de l'Université Laval"
+contribs = []
+refs = []
+abstracts = []
+
+[extra]
+container_name = "Un monde à tous et à personne"
+
+[ext_ids]
+doi = "10.2307/j.ctv27h1pd3.2"
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+[extra.crossref]
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Revision: 3ed7995c-0cbc-4640-b1dc-20e5d174cee0
--- (created)
+++ /release/rev/3ed7995c-0cbc-4640-b1dc-20e5d174cee0.toml
@@ -0,0 +1,20 @@
+title = "Avant-propos"
+work_id = "omp7xt7n2vbwbhmldtvwo3spxi"
+release_type = "chapter"
+release_stage = "published"
+release_date = "2021-12-17"
+release_year = 2021
+pages = "163-176"
+publisher = "Presses de l'Université Laval"
+contribs = []
+refs = []
+abstracts = []
+
+[extra]
+container_name = "Le Canada français"
+
+[ext_ids]
+doi = "10.2307/j.ctv27h1pg4.13"
+
+[extra.crossref]
+type = "book-chapter"
Revision: 43a85908-5b42-4100-9306-d6179cd473d9
--- (created)
+++ /release/rev/43a85908-5b42-4100-9306-d6179cd473d9.toml
@@ -0,0 +1,629 @@
+title = "Novel Macrocyclic Peptidomimetics Targeting the Polo-Box Domain of Polo-Like Kinase 1"
+work_id = "iejvqot6avffnih667u6s5ilwu"
+container_id = "biqbvvfsgzd3tgqsg6hhdhdk2u"
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+release_year = 2022
+publisher = "American Chemical Society (ACS)"
+language = "en"
+abstracts = []
+[[contribs]]
+index = 0
+raw_name = "SeongShick Ryu"
+given_name = "SeongShick"
+surname = "Ryu"
+role = "author"
+raw_affiliation = "KU-KIST Graduate School of Converging Science and Technology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea"
+
+[contribs.extra]
+more_affiliations = [ "Chemical Kinomics Research Center, Korea Institute of Science and Technology, 5 Hwarangro 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea", "Severance Biomedical Science Institute, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 03722, Republic of Korea",]
+seq = "first"
+[[contribs]]
+index = 1
+raw_name = "Jung-Eun Park"
+given_name = "Jung-Eun"
+surname = "Park"
+role = "author"
+raw_affiliation = "Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892, United States"
+
+[[contribs]]
+index = 2
+raw_name = "Young Jin Ham"
+given_name = "Young Jin"
+surname = "Ham"
+role = "author"
+raw_affiliation = "Chemical Kinomics Research Center, Korea Institute of Science and Technology, 5 Hwarangro 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea"
+
+[[contribs]]
+index = 3
+raw_name = "Daniel C. Lim"
+given_name = "Daniel C."
+surname = "Lim"
+role = "author"
+raw_affiliation = "Koch Institute for Integrative Cancer Research, and Center for Precision Cancer Medicine, Massachusetts Institute of Technology, 500 Main Street, Cambridge, Massachusetts 02139, United States"
+
+[[contribs]]
+index = 4
+raw_name = "Nicholas P. Kwiatkowski"
+given_name = "Nicholas P."
+surname = "Kwiatkowski"
+role = "author"
+raw_affiliation = "Harvard Medical School, 25 Shattuck Street, Boston, Massachusetts 02115, United States"
+
+[contribs.extra]
+more_affiliations = [ "Department of Cancer Biology, Dana-Farber Cancer Institute, 450 Brookline Avenue, Boston, Massachusetts 02215, United States",]
+[[contribs]]
+index = 5
+raw_name = "Do-Hee Kim"
+given_name = "Do-Hee"
+surname = "Kim"
+role = "author"
+raw_affiliation = "Chemical Kinomics Research Center, Korea Institute of Science and Technology, 5 Hwarangro 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea"
+
+[contribs.extra]
+more_affiliations = [ "Department of Chemistry, College of Convergence and Integrated Science, Kyonggi University, 154 Gwanggyosan-ro, Yeongtong-gu, Suwon 16227, Republic of Korea",]
+[[contribs]]
+index = 6
+raw_name = "Debabrata Bhunia"
+given_name = "Debabrata"
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+role = "author"
+raw_affiliation = "Chemical Kinomics Research Center, Korea Institute of Science and Technology, 5 Hwarangro 14-gil, Seongbuk-gu, Seoul 02792, Republic of Korea"
+
+[[contribs]]
+index = 7
+raw_name = "Nam Doo Kim"
+given_name = "Nam Doo"
+surname = "Kim"
+role = "author"
+raw_affiliation = "Voronoibio Inc., 32 Songdogwahak-ro, Yeonsu-gu, Incheon 21984, Republic of Korea"
+
+[[contribs]]
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+raw_name = "Michael B. Yaffe"
+given_name = "Michael B."
+surname = "Yaffe"
+role = "author"
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+content = "<jats:p>Chronic alcohol consumption leads to a spectrum of liver disease that is associated with significant global mortality and morbidity. Alcohol is known to deplete hepatic vitamin A content, which has been linked to the pathogenesis of alcoholic liver disease. It has been suggested that induction of Cytochrome P450 2E1 (CYP2E1) contributes to alcohol-induced hepatic vitamin A depletion, but the possible contributions of other retinoid-catabolizing CYPs have not been well studied. The main objective of this study was to better understand alcohol-induced hepatic vitamin A depletion and test the hypothesis that alcohol-induced depletion of hepatic vitamin A is due to CYP-mediated oxidative catabolism. This hypothesis was tested in a mouse model of chronic alcohol consumption, including wild type and <jats:italic>Cyp2e1</jats:italic> <jats:sup><jats:italic>-/-</jats:italic></jats:sup> mice. Our results show that chronic alcohol consumption is associated with decreased levels of hepatic retinol, retinyl esters, and retinoic acid. Moreover, the depletion of hepatic retinoid is associated with the induction of multiple retinoid catabolizing CYPs, including CYP26A1, and CYP26B1 in alcohol fed wild type mice. In <jats:italic>Cyp2e1</jats:italic> <jats:sup><jats:italic>-/-</jats:italic></jats:sup> mice, alcohol-induced retinol decline is blunted but retinyl esters undergo a change in their acyl composition and decline upon alcohol exposure like WT mice. In conclusion, the alcohol induced decline in hepatic vitamin A content is associated with increased expression of multiple retinoid-catabolizing CYPs, including the retinoic acid specific hydroxylases CYP26A1 and CYP26B1.</jats:p>"
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+year = 2020
+container_name = "Bioenergy Res"
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+
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+authors = [ "M Rokicka",]
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+index = 43
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+year = 2016
+container_name = "Resour Technol"
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+authors = [ "M Mubarak",]
+[[refs]]
+index = 44
+key = "pone.0262500.ref045"
+year = 2013
+container_name = "Biotechnol Biofuels"
+title = "Ultrasonic disintegration of microalgal biomass and consequent improvement of bioaccessibility/bioavailability in microbial fermentation."
+
+[refs.extra]
+authors = [ "BH Jeon",]
+[[refs]]
+index = 45
+key = "pone.0262500.ref046"
+year = 2017
+container_name = "Bioresour Technol"
+title = "Energy efficient bead milling of microalgae: Effect of bead size on disintegration and release of proteins and carbohydrates"
+locator = "670"
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+authors = [ "PR Postma",]
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+[[refs]]
+index = 46
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+year = 2014
+container_name = "Algal Res"
+title = "Release of hydro-soluble microalgal proteins using mechanical and chemical treatments"
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+authors = [ "C Safi",]
+doi = "10.1016/j.algal.2013.11.017"
+[[refs]]
+index = 47
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+
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+index = 48
+key = "pone.0262500.ref049"
+year = 2015
+container_name = "Algal Res"
+title = "Nitrogen-to-protein conversion factors revisited for applications of microalgal biomass conversion to food, feed and fuel."
+locator = "359"
+
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+authors = [ "DW Templeton",]
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+[[refs]]
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+year = 2014
+container_name = "Water Res"
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+locator = "350"
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+container_name = "Algal Res"
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+[[refs]]
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+year = 2002
+container_name = "J Agric Food Chem"
+title = "Lignin deposition and associated changes in anatomy, enzyme activity, gene expression, and ruminal degradability in stems of tall fescue at different developmental stages"
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+[[refs]]
+index = 52
+key = "pone.0262500.ref053"
+year = 2000
+container_name = "Phytochemistry"
+title = "Comparison of neutral lipid profile of various trilaminar outer cell wall (TLS)-containing microalgae with emphasis on algaenan occurrence."
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+
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+authors = [ "B Allard",]
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+volume = "54"
+[[refs]]
+index = 53
+key = "pone.0262500.ref054"
+year = 2009
+container_name = "Org Geochem"
+title = "Phylogenetic investigation of the aliphatic, non-hydrolyzable biopolymer algaenan, with a focus on green algae"
+locator = "854"
+
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+authors = [ "RB Kodner",]
+doi = "10.1016/j.orggeochem.2009.05.003"
+volume = "40"
+[[refs]]
+index = 54
+key = "pone.0262500.ref055"
+year = 1995
+title = "Insoluble, Nonhydrolyzable, Aliphatic Macromolecular Constituents of Microbial Cell Walls."
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+
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+authors = [ "C Largeau",]
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+[[refs]]
+index = 55
+key = "pone.0262500.ref056"
+year = 2018
+container_name = "Front Microbiol"
+title = "Cell wall structure of coccoid green algae as an important trade-off between biotic interference mechanisms and multidimensional cell growth"
+
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+authors = [ "S Dunker",]
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+[[refs]]
+index = 56
+key = "pone.0262500.ref057"
+year = 1945
+container_name = "Ind Eng Chem"
+title = "Kinetics of Wood Saccharification—Hydrolysis of Cellulose and Decomposition of Sugars in Dilute Acid at High Temperature."
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+
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+authors = [ "JF Saeman",]
+doi = "10.1021/ie50421a009"
+volume = "37"
+[[refs]]
+index = 57
+key = "pone.0262500.ref058"
+year = 2021
+container_name = "J Agric Food Chem"
+title = "Biochemical and morphological characterization of heterotrophic crypthecodinium cohnii and Chlorella vulgaris cell walls"
+locator = "2226"
+
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+authors = [ "A Mathys",]
+doi = "10.1021/acs.jafc.0c05032"
+volume = "69"
+[[refs]]
+index = 58
+key = "pone.0262500.ref059"
+year = 2014
+container_name = "Renew Sustain Energy Rev"
+title = "Morphology, composition, production, processing and applications of Chlorella vulgaris: A review."
+locator = "265"
+
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+authors = [ "C Safi",]
+doi = "10.1016/j.rser.2014.04.007"
+volume = "35"
+[[refs]]
+index = 59
+key = "pone.0262500.ref060"
+year = 2001
+container_name = "J Appl Glycosci"
+title = "A New.BETA.-D-Galactan Having 3-O-Methyl-D-galactose from Chlorella vulgaris."
+locator = "325"
+
+[refs.extra]
+authors = [ "K Ogawa",]
+doi = "10.5458/jag.48.325"
+volume = "48"
+[[refs]]
+index = 60
+key = "pone.0262500.ref061"
+year = 1996
+container_name = "Planta Med"
+title = "A water-soluble antitumor glycoprotein from Chlorella vulgaris"
+locator = "423"
+
+[refs.extra]
+authors = [ "K Noda",]
+doi = "10.1055/s-2006-957931"
+volume = "62"
+[[refs]]
+index = 61
+key = "pone.0262500.ref062"
+year = 2021
+container_name = "Lwt"
+title = "Tailored enzymatic treatment of Chlorella vulgaris cell wall leads to effective disruption while preserving oxidative stability"
+locator = "111157"
+
+[refs.extra]
+authors = [ "G Canelli",]
+doi = "10.1016/j.lwt.2021.111157"
+issue = "February"
+volume = "143"
+[[refs]]
+index = 62
+key = "pone.0262500.ref063"
+year = 2020
+container_name = "Algal Res"
+title = "Reserve, structural and extracellular polysaccharides of Chlorella vulgaris: A holistic approach."
+locator = "101757"
+
+[refs.extra]
+authors = [ "AS Ferreira",]
+doi = "10.1016/j.algal.2019.101757"
+volume = "45"
+[[refs]]
+index = 63
+key = "pone.0262500.ref064"
+year = 2021
+container_name = "Iran J Sci Technol Trans A Sci"
+title = "Efficient Saccharification of the Microalga Chlorella vulgaris and its Conversion into Ethanol by Fermentation."
+locator = "767"
+
+[refs.extra]
+authors = [ "GW Abou El-Souod",]
+doi = "10.1007/s40995-021-01097-1"
+volume = "45"
+[[refs]]
+index = 64
+key = "pone.0262500.ref065"
+year = 2019
+container_name = "Sci Rep"
+title = "Novel combination of feed enzymes to improve the degradation of Chlorella vulgaris recalcitrant cell wall"
+
+[refs.extra]
+authors = [ "D Coelho",]
+doi = "10.1038/s41598-019-41775-0"
+[[refs]]
+index = 65
+key = "pone.0262500.ref066"
+year = 1980
+container_name = "Plant Cell Physiol"
+title = "Alkalinity-induced aggregation in Chlorella vulgaris I. Changes in cell volume and cell-wall structure"
+locator = "27"
+
+[refs.extra]
+authors = [ "S Malis-Arad",]
+volume = "21"
+[[abstracts]]
+sha1 = "36eaa2ab23e0912f80fb3bd6010792fd387cb182"
+content = "<jats:p>With their ability of CO<jats:sub>2</jats:sub> fixation using sunlight as an energy source, algae and especially microalgae are moving into the focus for the production of proteins and other valuable compounds. However, the valorization of algal biomass depends on the effective disruption of the recalcitrant microalgal cell wall. Especially cell walls of <jats:italic>Chlorella</jats:italic> species proved to be very robust. The wall structures that are responsible for this robustness have been studied less so far. Here, we evaluate different common methods to break up the algal cell wall effectively and measure the success by protein and carbohydrate release. Subsequently, we investigate algal cell wall features playing a role in the wall's recalcitrance towards disruption. Using different mechanical and chemical technologies, alkali catalyzed hydrolysis of the <jats:italic>Chlorella vulgaris</jats:italic> cells proved to be especially effective in solubilizing up to 56 wt% protein and 14 wt% carbohydrates of the total biomass. The stepwise degradation of <jats:italic>C</jats:italic>. <jats:italic>vulgaris</jats:italic> cell walls using a series of chemicals with increasingly strong conditions revealed that each fraction released different ratios of proteins and carbohydrates. A detailed analysis of the monosaccharide composition of the cell wall extracted in each step identified possible factors for the robustness of the cell wall. In particular, the presence of chitin or chitin-like polymers was indicated by glucosamine found in strong alkali extracts. The presence of highly ordered starch or cellulose was indicated by glucose detected in strong acidic extracts. Our results might help to tailor more specific efforts to disrupt <jats:italic>Chlorella</jats:italic> cell walls and help to valorize microalgae biomass.</jats:p>"
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+content = "<jats:p>Abstract We present a case of Sarcoptes and canine distemper virus (CDV) infection in a white-nosed coati (Nasua narica) that was trapped in the dry tropical forest of Cerro Blanco reserve, located in the coastal region of Ecuador. Sarcoptic mange is a highly contagious and zoonotic disease with worldwide distribution that causes epidemics. Mange is produced by Sarcoptes mites that causes severe epidermal damage. Secondary infections and physiological constrictions without treatment can lead to death of the host. In addition, cooccurrence of canine distemper virus was detected via iiRT-PCR from serum samples. Physical analyses showed that 90% of the skin was affected by severe alopecia due to the sarcoptic mange infection. The presence of mites and histopathological analyses confirmed the diagnosis of infection. This coati was taken to a veterinary clinic and was fed every day, but it died after four days. This is the first report of sarcoptic mange and the first report of CDV in white-nosed coatis in South America. Further studies are needed in this region, to seek out other suspected cases, given the high capacity for disease transmission. Preventive actions to avoid epidemic and zoonotic episodes are needed.</jats:p>"
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